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| 001 | 137186 | ||
| 005 | 20240321220215.0 | ||
| 024 | 7 | _ | |a 10.1042/BJ20130788 |2 doi |
| 024 | 7 | _ | |a pmid:24059268 |2 pmid |
| 024 | 7 | _ | |a 0006-2936 |2 ISSN |
| 024 | 7 | _ | |a 0264-6021 |2 ISSN |
| 024 | 7 | _ | |a 0306-3275 |2 ISSN |
| 024 | 7 | _ | |a 0306-3283 |2 ISSN |
| 024 | 7 | _ | |a 1470-8728 |2 ISSN |
| 024 | 7 | _ | |a altmetric:1778220 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-03508 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 540 |
| 100 | 1 | _ | |a Wirth, Alexander |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Dual lipidation of the brain-specific Cdc42 isoform regulates its functional properties. |
| 260 | _ | _ | |a London |c 2013 |b Portland Press67261 |
| 264 | _ | 1 | |3 online |2 Crossref |b Portland Press Ltd. |c 2013-11-22 |
| 264 | _ | 1 | |3 print |2 Crossref |b Portland Press Ltd. |c 2013-12-15 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1586266615_6465 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Cdc42 (cell division cycle 42) is a member of the Rho GTPase family which regulates a variety of cellular activities by controlling actin cytoskeleton and gene expression. Cdc42 is expressed in the form of two splice variants. The canonical Cdc42 isoform is prenylated (Cdc42-prenyl), whereas the brainspecific isoform can be palmitoylated (Cdc42-palm). In the present study we have demonstrated palmitoylation of endogenous Cdc42 in rodent and human brains and identified Cys(188) and Cys(189) as acylation sites of Cdc42-palm. Moreover, we have shown that Cys(188) can also be prenylated. Analysis of acylation-deficient mutants revealed that lipidation of Cys(188) is essential for proper membrane binding of Cdc42-palm as well as for Cdc42-mediated regulation of gene transcription and induction of densely packed filopodia in neuroblastoma cells. We also found that Cdc42-prenyl is a dominant splice variant in a wide range of commonly used cell lines as well as in the cerebellum, whereas Cdc42-palm is the main Cdc42 isoform in hippocampus, where it is critically involved in the formation of dendritic filopodia and spines. Replacement of endogenous Cdc42 by its acylation-deficient mutants revealed the importance of Cdc42-palm lipidation for its morphogenic and synaptogenic effects in neurons. These findings demonstrate that dual lipidation of Cdc42-palm represents an important regulator of morphogenic signalling in hippocampal neurons. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Cdc42 protein, mouse |2 NLM Chemicals |
| 650 | _ | 7 | |a Isoenzymes |2 NLM Chemicals |
| 650 | _ | 7 | |a cdc42 GTP-Binding Protein |0 EC 3.6.5.2 |2 NLM Chemicals |
| 650 | _ | 7 | |a Cysteine |0 K848JZ4886 |2 NLM Chemicals |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Cell Line, Tumor |2 MeSH |
| 650 | _ | 2 | |a Cerebellum: cytology |2 MeSH |
| 650 | _ | 2 | |a Cerebellum: metabolism |2 MeSH |
| 650 | _ | 2 | |a Cysteine: genetics |2 MeSH |
| 650 | _ | 2 | |a Cysteine: metabolism |2 MeSH |
| 650 | _ | 2 | |a Dendrites: genetics |2 MeSH |
| 650 | _ | 2 | |a Dendrites: metabolism |2 MeSH |
| 650 | _ | 2 | |a Hippocampus: cytology |2 MeSH |
| 650 | _ | 2 | |a Hippocampus: metabolism |2 MeSH |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Isoenzymes: genetics |2 MeSH |
| 650 | _ | 2 | |a Isoenzymes: metabolism |2 MeSH |
| 650 | _ | 2 | |a Lipoylation: physiology |2 MeSH |
| 650 | _ | 2 | |a Mice |2 MeSH |
| 650 | _ | 2 | |a Organ Specificity: physiology |2 MeSH |
| 650 | _ | 2 | |a Protein Prenylation: physiology |2 MeSH |
| 650 | _ | 2 | |a Pseudopodia: genetics |2 MeSH |
| 650 | _ | 2 | |a Pseudopodia: metabolism |2 MeSH |
| 650 | _ | 2 | |a Transcription, Genetic: physiology |2 MeSH |
| 650 | _ | 2 | |a cdc42 GTP-Binding Protein: genetics |2 MeSH |
| 650 | _ | 2 | |a cdc42 GTP-Binding Protein: metabolism |2 MeSH |
| 700 | 1 | _ | |a Chen-Wacker, Chen |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Wu, Yao-Wen |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Gorinski, Nataliya |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Filippov, Mikhail A |0 P:(DE-2719)2810721 |b 4 |u dzne |
| 700 | 1 | _ | |a Pandey, Ghanshyam |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Ponimaskin, Evgeni |0 P:(DE-HGF)0 |b 6 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1042/bj20130788 |b : Portland Press Ltd., 2013-11-22 |n 3 |p 311-322 |3 journal-article |2 Crossref |t Biochemical Journal |v 456 |y 2013 |x 0264-6021 |
| 773 | _ | _ | |a 10.1042/BJ20130788 |g Vol. 456, no. 3, p. 311 - 322 |0 PERI:(DE-600)1473095-9 |n 3 |q 456:3<311 - 322 |p 311-322 |t Biochemical journal |v 456 |y 2013 |x 0264-6021 |
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| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 4 |6 P:(DE-2719)2810721 |
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