engCastillo-Gomez, EstherKästner, AnneSchmidt, UlrikePfander, ViktoriaHammer, ChristianSchulz, Thomas FBinder, LutzStöcker, WinfriedWeber, FrankEhrenreich, HanneloreSteiner, JohannSchneider, AnjaHettling, BilkePoggi, GiuliaOstehr, KristinUhr, ManfredAsif, Abdul RMatzke, MikeThe brain as immunoprecipitator of serum autoantibodies against N-Methyl-D-aspartate receptor subunit NR1.info:eu-repo/classification/ddc/610AdultAnimalsAutoantibodies: bloodAutoantibodies: cerebrospinal fluidBlood-Brain Barrier: physiopathologyBrain: immunologyFemaleHumansMaleMiceMice, Inbred C57BLMiddle AgedMultiple Sclerosis: bloodMultiple Sclerosis: cerebrospinal fluidReceptors, N-Methyl-D-Aspartate: immunologySeroepidemiologic StudiesAutoantibodiesNR1 NMDA receptorReceptors, N-Methyl-D-AspartateAutoantibodies (AB) against N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) are highly seroprevalent in health and disease. Symptomatic relevance may arise upon compromised blood-brain barrier (BBB). However, it remained unknown whether circulating NMDAR1 AB appear in the cerebrospinal fluid (CSF). Of n = 271 subjects with CSF-serum pairs, 26 were NMDAR1 AB seropositive, but only 1 was CSF positive. Contrariwise, tetanus AB (non-brain-binding) were present in serum and CSF of all subjects, with CSF levels higher upon BBB dysfunction. Translational mouse experiments proved the hypothesis that the brain acts as an 'immunoprecipitator'; simultaneous injection of NMDAR1 AB and the non-brain-binding green fluorescent protein AB resulted in high detectability of the former in brain and the latter in CSF.Annals of neurology 79(1), 144-151 (2016). doi:10.1002/ana.24545info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionWiley-Blackwell2016info:eu-repo/semantics/closedAccessDEhttps://pub.dzne.de/record/138393https://pub.dzne.de/search?p=id:%22DZNE-2020-04715%22Researchersinfo:eu-repo/semantics/altIdentifier/pmid/pmid:26505629info:eu-repo/semantics/altIdentifier/issn/0364-5134info:eu-repo/semantics/altIdentifier/issn/1531-8249info:eu-repo/semantics/altIdentifier/doi/10.1002/ana.24545