138817 20240321220513.0 10.1038/nmeth.3964 doi pmid:27548807 pmid 1548-7091 ISSN 1548-7105 ISSN altmetric:10759133 altmetric DZNE-2020-05139 English 610 Pan, Chenchen P:(DE-HGF)0 0 Shrinkage-mediated imaging of entire organs and organisms using uDISCO. London [u.a.] 2016 Nature Publishing Group online Crossref Springer Science and Business Media LLC 2016-08-22 print Crossref Springer Science and Business Media LLC 2016-10-01 article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1585579061_26570 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Recent tissue-clearing approaches have become important alternatives to standard histology approaches. However, light scattering in thick tissues and the size restrictions on samples that can be imaged with standard light-sheet microscopy pose limitations for analyzing large samples such as an entire rodent body. We developed 'ultimate DISCO' (uDISCO) clearing to overcome these limitations in volumetric imaging. uDISCO preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65%. We used uDISCO to image neuronal connections and vasculature from head to toe over 7 cm and to perform unbiased screening of transplanted stem cells within the entire body of adult mice. uDISCO is compatible with diverse labeling methods and archival human tissue, and it can readily be used in various biomedical applications to study organization of large organ systems throughout entire organisms. 344 - Clinical and Health Care Research (POF3-344) G:(DE-HGF)POF3-344 POF3-344 POF III 0 2016-08-22 Crossref http://www.springer.com/tdm Dataset connected to CrossRef, PubMed, Contrast Media NLM Chemicals Phenyl Ethers NLM Chemicals Solvents NLM Chemicals Green Fluorescent Proteins 147336-22-9 NLM Chemicals phenyl ether 3O695R5M1U NLM Chemicals Animals MeSH Central Nervous System: blood supply MeSH Central Nervous System: cytology MeSH Contrast Media MeSH Female MeSH Green Fluorescent Proteins: analysis MeSH Green Fluorescent Proteins: chemistry MeSH Green Fluorescent Proteins: genetics MeSH Half-Life MeSH Humans MeSH Imaging, Three-Dimensional: methods MeSH Immunohistochemistry: methods MeSH Male MeSH Mice, Inbred C57BL MeSH Mice, Transgenic MeSH Microscopy, Fluorescence: methods MeSH Neuroimaging: methods MeSH Organ Specificity MeSH Phenyl Ethers: chemistry MeSH Rats MeSH Single-Cell Analysis: methods MeSH Solvents: chemistry MeSH Staining and Labeling MeSH Whole Body Imaging: methods MeSH Cai, Ruiyao P:(DE-HGF)0 1 Quacquarelli, Francesca Paola P:(DE-HGF)0 2 Ghasemigharagoz, Alireza P:(DE-HGF)0 3 Lourbopoulos, Athanasios P:(DE-HGF)0 4 Matryba, Paweł P:(DE-HGF)0 5 Plesnila, Nikolaus P:(DE-HGF)0 6 Dichgans, Martin P:(DE-2719)2000030 7 dzne Hellal, Farida P:(DE-HGF)0 8 Ertürk, Ali P:(DE-HGF)0 9 Corresponding author 10.1038/nmeth.3964 : Springer Science and Business Media LLC, 2016-08-22 10 859-867 journal-article Crossref Nature Methods 13 2016 1548-7091 10.1038/nmeth.3964 Vol. 13, no. 10, p. 859 - 867 PERI:(DE-600)2163081-1 10 13:10<859 - 867 859-867 Nature methods 13 2016 1548-7091 oai:pub.dzne.de:138817 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 7 P:(DE-2719)2000030 DE-HGF Forschungsbereich Gesundheit Erkrankungen des Nervensystems G:(DE-HGF)POF3-340 G:(DE-HGF)POF3-344 G:(DE-HGF)POF3-300 Clinical and Health Care Research 0 2016 Nationallizenz StatID:(DE-HGF)0420 StatID DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS DBCoverage StatID:(DE-HGF)0300 StatID Medline DBCoverage StatID:(DE-HGF)0310 StatID NCBI Molecular Biology Database JCR StatID:(DE-HGF)0100 StatID NAT METHODS : 2017 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search Peer Review StatID:(DE-HGF)0030 StatID ASC DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List WoS StatID:(DE-HGF)0110 StatID Science Citation Index DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection WoS StatID:(DE-HGF)0111 StatID Science Citation Index Expanded DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - 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