138902 20240321220522.0 10.1038/cdd.2016.79 doi pmid:27518434 pmid pmc:PMC5136489 pmc 1350-9047 ISSN 1476-5403 ISSN altmetric:10474005 altmetric DZNE-2020-05224 English 610 Körver, Lisa P:(DE-2719)2810874 0 First author dzne The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila. London 2016 Macmillan online Crossref Springer Science and Business Media LLC 2016-08-12 print Crossref Springer Science and Business Media LLC 2016-12-01 article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1592980663_30012 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote De-ubiquitylating enzymes (DUBs) reverse protein ubiquitylation and thereby control essential cellular functions. Screening for a DUB that counteracts caspase ubiquitylation to regulate cell survival, we identified the Drosophila ovarian tumour-type DUB DUBA (CG6091). DUBA physically interacts with the initiator caspase death regulator Nedd2-like caspase (Dronc) and de-ubiquitylates it, thereby contributing to efficient inhibitor of apoptosis-antagonist-induced apoptosis in the fly eye. Searching also for non-apoptotic functions of DUBA, we found that Duba-null mutants are male sterile and display defects in spermatid individualisation, a process that depends on non-apoptotic caspase activity. Spermatids of DUBA-deficient flies showed reduced caspase activity and lack critical structures of the individualisation process. Biochemical characterisation revealed an obligate activation step of DUBA by phosphorylation. With genetic rescue experiments we demonstrate that DUBA phosphorylation and catalytic activity are crucial in vivo for DUBA function in spermatogenesis. Our results demonstrate for the first time the importance of de-ubiquitylation for fly spermatogenesis. 341 - Molecular Signaling (POF3-341) G:(DE-HGF)POF3-341 POF3-341 POF III 0 2016-08-12 Crossref http://www.springer.com/tdm Dataset connected to CrossRef, PubMed, Drosophila Proteins NLM Chemicals Ubiquitinated Proteins NLM Chemicals Phosphoserine 17885-08-4 NLM Chemicals DUBA protein, Drosophila EC 3.4.19.12 NLM Chemicals Deubiquitinating Enzymes EC 3.4.19.12 NLM Chemicals Caspases EC 3.4.22.- NLM Chemicals Amino Acid Sequence MeSH Animals MeSH Apoptosis MeSH Biocatalysis MeSH Caspases: metabolism MeSH Deubiquitinating Enzymes: chemistry MeSH Deubiquitinating Enzymes: metabolism MeSH Drosophila Proteins: chemistry MeSH Drosophila Proteins: metabolism MeSH Drosophila melanogaster: enzymology MeSH Drosophila melanogaster: physiology MeSH Male MeSH Phosphorylation MeSH Phosphoserine: metabolism MeSH Protein Binding MeSH Spermatogenesis MeSH Testis: metabolism MeSH Ubiquitinated Proteins: metabolism MeSH Ubiquitination MeSH Melzer, Juliane P:(DE-2719)2810805 1 dzne Aguado Roca, Eva P:(DE-2719)2810320 2 dzne Teichert, Dominic P:(DE-2719)2810393 3 dzne Glatter, Timo P:(DE-HGF)0 4 Arama, Eli P:(DE-HGF)0 5 Broemer, Meike P:(DE-2719)2810307 6 Last author dzne 10.1038/cdd.2016.79 : Springer Science and Business Media LLC, 2016-08-12 12 2019-2030 journal-article Crossref Cell Death & Differentiation 23 2016 1350-9047 10.1038/cdd.2016.79 Vol. 23, no. 12, p. 2019 - 2030 PERI:(DE-600)1496681-5 12 23:12<2019 - 2030 2019-2030 Cell death and differentiation 23 2016 1350-9047 Pubmed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5136489 oai:pub.dzne.de:138902 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)2810874 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)2810805 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)2810320 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)2810393 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)2810307 DE-HGF Forschungsbereich Gesundheit Erkrankungen des Nervensystems G:(DE-HGF)POF3-340 G:(DE-HGF)POF3-341 G:(DE-HGF)POF3-300 Molecular Signaling 0 2016 JCR StatID:(DE-HGF)0100 StatID CELL DEATH DIFFER : 2017 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS DBCoverage StatID:(DE-HGF)0300 StatID Medline DBCoverage StatID:(DE-HGF)0310 StatID NCBI Molecular Biology Database DBCoverage StatID:(DE-HGF)0320 StatID PubMed Central DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search Peer Review StatID:(DE-HGF)0030 StatID ASC DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List WoS StatID:(DE-HGF)0110 StatID Science Citation Index DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection WoS StatID:(DE-HGF)0111 StatID Science Citation Index Expanded DBCoverage 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