140925 20240321220913.0 10.1371/journal.pbio.3000374 doi pmid:31393866 pmid pmc:PMC6701817 pmc 1544-9173 ISSN 1545-7885 ISSN altmetric:64732725 altmetric DZNE-2020-07247 English 610 Kaczmarczyk, Lech P:(DE-2719)2810486 0 First author dzne Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. Lawrence, KS 2019 PLoS online Crossref Public Library of Science (PLoS) 2019-08-08 article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1604063435_512 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line. 342 - Disease Mechanisms and Model Systems (POF3-342) G:(DE-HGF)POF3-342 POF3-342 POF III 0 2019-08-08 Crossref http://creativecommons.org/licenses/by/4.0/ Dataset connected to CrossRef, PubMed, Animals MeSH Cloning, Molecular: methods MeSH Gene Expression: genetics MeSH Gene Expression Profiling: methods MeSH Gene Expression Regulation: genetics MeSH Gene Knock-In Techniques MeSH Genomics: methods MeSH Mice MeSH Mice, Inbred C57BL MeSH Mice, Transgenic: genetics MeSH MicroRNAs: genetics MeSH Nucleic Acids: isolation & purification MeSH Proteomics: methods MeSH RNA, Messenger: genetics MeSH Transcriptome: genetics MeSH Transgenes: genetics MeSH Whole Genome Sequencing: methods MeSH Bansal, Vikas P:(DE-HGF)0 1 Rajput, Ashish P:(DE-HGF)0 2 Rahman, Raza-Ur P:(DE-HGF)0 3 Krzyżak, Wiesław P:(DE-HGF)0 4 Degen, Joachim P:(DE-HGF)0 5 Poll, Stefanie P:(DE-2719)2810397 6 dzne Fuhrmann, Martin P:(DE-2719)2679991 7 dzne Bonn, Stefan P:(DE-2719)2810547 8 dzne Jackson, Walker Scot P:(DE-2719)2810253 9 Last author dzne 10.1371/journal.pbio.3000374 : Public Library of Science (PLoS), 2019-08-08 8 e3000374 journal-article Crossref PLOS Biology 17 2019 1545-7885 10.1371/journal.pbio.3000374 Vol. 17, no. 8, p. e3000374 - PERI:(DE-600)2126773-X 8 17:8<e3000374 - e3000374 PLoS biology 17 2019 1545-7885 https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000374 Pubmed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701817 http://pub.dzne.de/record/140925/files/3222.pdf OpenAccess http://pub.dzne.de/record/140925/files/3222.gif?subformat=icon icon OpenAccess http://pub.dzne.de/record/140925/files/3222.jpg?subformat=icon-1440 icon-1440 OpenAccess http://pub.dzne.de/record/140925/files/3222.jpg?subformat=icon-180 icon-180 OpenAccess http://pub.dzne.de/record/140925/files/3222.jpg?subformat=icon-640 icon-640 OpenAccess http://pub.dzne.de/record/140925/files/3222.pdf?subformat=pdfa pdfa OpenAccess oai:pub.dzne.de:140925 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)2810486 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)2810397 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 7 P:(DE-2719)2679991 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 8 P:(DE-2719)2810547 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 9 P:(DE-2719)2810253 DE-HGF Forschungsbereich Gesundheit Erkrankungen des Nervensystems G:(DE-HGF)POF3-340 G:(DE-HGF)POF3-342 G:(DE-HGF)POF3-300 Disease Mechanisms and Model Systems 0 2019 Creative Commons Attribution CC BY (No Version) LIC:(DE-HGF)CCBYNV V:(DE-HGF) DOAJ OpenAccess StatID:(DE-HGF)0510 StatID DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2022-11-11 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2022-11-11 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2022-01-10T10:28:08Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2022-01-10T10:28:08Z Peer Review StatID:(DE-HGF)0030 StatID DOAJ : Blind peer review 2022-01-10T10:28:08Z DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2022-11-11 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2022-11-11 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2022-11-11 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2022-11-11 DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2022-11-11 DBCoverage StatID:(DE-HGF)1060 StatID Current Contents - Agriculture, Biology and Environmental Sciences 2022-11-11 JCR StatID:(DE-HGF)0100 StatID PLOS BIOL : 2021 2022-11-11 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2022-11-11 Peer Review StatID:(DE-HGF)0030 StatID ASC 2022-11-11 IF >= 5 StatID:(DE-HGF)9905 StatID PLOS BIOL : 2021 2022-11-11 I:(DE-2719)1013019 AG Jackson Selective Vulnerability of Neurodegenerative Diseases 0 I:(DE-2719)1011004 AG Fuhrmann Neuroimmunology and Imaging 1 I:(DE-2719)1410003 AG Bonn 1 Computational analysis of biological networks 2 journal VDB UNRESTRICTED I:(DE-2719)1013019 I:(DE-2719)1011004 I:(DE-2719)1410003 FullTexts -- missing cx lookup -- 10.1073/pnas.0408031102 Crossref 10.1073/pnas.0408031102 2015 Crossref Molecular biology of the cell B. Alberts Molecular biology of the cell 2015 2018 Crossref JWB Hershey 2018 -- missing cx lookup -- 10.1038/nature07228 Crossref 10.1038/nature07228 -- missing cx lookup -- 10.1016/j.cell.2018.03.006 Crossref 10.1016/j.cell.2018.03.006 -- missing cx lookup -- 10.1007/s00441-017-2711-z Crossref 10.1007/s00441-017-2711-z -- missing cx lookup -- 10.1534/genetics.112.146704 Crossref 10.1534/genetics.112.146704 2015 Crossref L Kaczmarczyk 2015 -- missing cx lookup -- 10.1016/j.cell.2008.10.029 Crossref 10.1016/j.cell.2008.10.029 -- missing cx lookup -- 10.1073/pnas.0907143106 Crossref 10.1073/pnas.0907143106 -- missing cx lookup -- 10.1016/j.neuron.2011.11.010 Crossref 10.1016/j.neuron.2011.11.010 -- missing cx lookup -- 10.1084/jem.20100451 Crossref 10.1084/jem.20100451 -- missing cx lookup -- 10.1038/nprot.2014.023 Crossref 10.1038/nprot.2014.023 -- missing cx lookup -- 10.1101/gad.205278.112 Crossref 10.1101/gad.205278.112 -- missing cx lookup -- 10.1016/j.neuron.2015.05.018 Crossref 10.1016/j.neuron.2015.05.018 -- missing cx lookup -- 10.1016/j.cell.2012.11.022 Crossref 10.1016/j.cell.2012.11.022 -- missing cx lookup -- 10.1186/1471-2202-9-42 Crossref 10.1186/1471-2202-9-42 -- missing cx lookup -- 10.1016/j.celrep.2016.12.087 Crossref 10.1016/j.celrep.2016.12.087 -- missing cx lookup -- 10.1038/nbt957 Crossref 10.1038/nbt957 -- missing cx lookup -- 10.1371/journal.pone.0018556 Crossref 10.1371/journal.pone.0018556 -- missing cx lookup -- 10.1038/nn.2467 Crossref 10.1038/nn.2467 -- missing cx lookup -- 10.1016/j.neuron.2015.02.022 Crossref 10.1016/j.neuron.2015.02.022 -- missing cx lookup -- 10.1038/s41598-017-02460-2 Crossref 10.1038/s41598-017-02460-2 -- missing cx lookup -- 10.1016/j.neuron.2011.05.028 Crossref 10.1016/j.neuron.2011.05.028 -- missing cx lookup -- 10.1016/j.neuron.2011.07.026 Crossref 10.1016/j.neuron.2011.07.026 -- missing cx lookup -- 10.1371/journal.pbio.0030159 Crossref 10.1371/journal.pbio.0030159 -- missing cx lookup -- 10.1186/s13059-014-0550-8 Crossref 10.1186/s13059-014-0550-8 -- missing cx lookup -- 10.1186/s12859-018-2047-z Crossref 10.1186/s12859-018-2047-z -- missing cx lookup -- 10.1126/science.aao2793 Crossref 10.1126/science.aao2793 -- missing cx lookup -- 10.1126/science.aam8526 Crossref 10.1126/science.aam8526 -- missing cx lookup -- 10.1016/j.cell.2018.05.022 Crossref 10.1016/j.cell.2018.05.022 -- missing cx lookup -- 10.1038/nmeth.2483 Crossref 10.1038/nmeth.2483 -- missing cx lookup -- 10.1073/pnas.1720956115 Crossref 10.1073/pnas.1720956115 -- missing cx lookup -- 10.1038/nn.4194 Crossref 10.1038/nn.4194 -- missing cx lookup -- 10.1016/j.cell.2014.03.042 Crossref 10.1016/j.cell.2014.03.042 -- missing cx lookup -- 10.1038/nprot.2012.049 Crossref 10.1038/nprot.2012.049 -- missing cx lookup -- 10.1042/BJ20081949 Crossref 10.1042/BJ20081949 -- missing cx lookup -- 10.1038/ncomms5450 Crossref 10.1038/ncomms5450 -- missing cx lookup -- 10.1111/ejn.12506 Crossref 10.1111/ejn.12506 -- missing cx lookup -- 10.1083/jcb.201601004 Crossref 10.1083/jcb.201601004 -- missing cx lookup -- 10.1038/nn.4216 Crossref 10.1038/nn.4216 -- missing cx lookup -- 10.1038/s41593-018-0221-2 Crossref 10.1038/s41593-018-0221-2 -- missing cx lookup -- 10.1242/dmm.012146 Crossref 10.1242/dmm.012146 -- missing cx lookup -- 10.1016/j.cell.2013.02.014 Crossref 10.1016/j.cell.2013.02.014 -- missing cx lookup -- 10.1016/j.neuron.2017.09.057 Crossref 10.1016/j.neuron.2017.09.057 -- missing cx lookup -- 10.4172/2161-0460.1000420 Crossref 10.4172/2161-0460.1000420 -- missing cx lookup -- 10.1101/gad.5.9.1513 Crossref 10.1101/gad.5.9.1513 -- missing cx lookup -- 10.1038/nrg1606 Crossref 10.1038/nrg1606 -- missing cx lookup -- 10.1111/cns.12655 Crossref 10.1111/cns.12655 -- missing cx lookup -- 10.1038/nmeth.4435 Crossref 10.1038/nmeth.4435 -- missing cx lookup -- 10.1038/nmeth.4582 Crossref 10.1038/nmeth.4582 -- missing cx lookup -- 10.1016/j.molcel.2017.01.023 Crossref 10.1016/j.molcel.2017.01.023 -- missing cx lookup -- 10.1371/journal.pcbi.1002690 Crossref 10.1371/journal.pcbi.1002690 -- missing cx lookup -- 10.1006/mthe.2000.0050 Crossref 10.1006/mthe.2000.0050 -- missing cx lookup -- 10.1261/rna.1229409 Crossref 10.1261/rna.1229409 -- missing cx lookup -- 10.1016/j.molcel.2004.07.007 Crossref 10.1016/j.molcel.2004.07.007 -- missing cx lookup -- 10.1016/0378-1119(91)90434-D Crossref 10.1016/0378-1119(91)90434-D -- missing cx lookup -- 10.1038/nbt0502-455 Crossref 10.1038/nbt0502-455 -- missing cx lookup -- 10.1371/journal.pone.0154604 Crossref 10.1371/journal.pone.0154604 -- missing cx lookup -- 10.1038/75973 Crossref 10.1038/75973 -- missing cx lookup -- 10.1371/journal.pone.0000162 Crossref 10.1371/journal.pone.0000162 -- missing cx lookup -- 10.1023/A:1008868325009 Crossref 10.1023/A:1008868325009 -- missing cx lookup -- 10.1073/pnas.1707337115 Crossref 10.1073/pnas.1707337115 -- missing cx lookup -- 10.1002/cpch.12 Crossref 10.1002/cpch.12 -- missing cx lookup -- 10.1093/bioinformatics/btr671 Crossref 10.1093/bioinformatics/btr671 -- missing cx lookup -- 10.1093/nar/gkw257 Crossref 10.1093/nar/gkw257