141160 20250416155312.0 10.1002/hipo.22399 doi pmid:25483308 pmid 1050-9631 ISSN 1098-1063 ISSN altmetric:2967229 altmetric DZNE-2020-07482 English 610 Turimella, Sada Lakshmi 0 Characterization of cytoplasmic polyadenylation element binding 2 protein expression and its RNA binding activity. New York, NY [u.a.] 2015 Wiley online Crossref Wiley 2014-12-23 print Crossref Wiley 2015-05-01 article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1744811549_2859 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Cytoplasmic polyadenylation element binding (CPEB) proteins are translational regulators that are involved in the control of cellular senescence, synaptic plasticity, learning, and memory. We have previously found all four known CPEB family members to be transcribed in the mouse hippocampus. Aside from a brief description of CPEB2 in mouse brain, not much is known about its biological role. Hence, this study aims to investigate CPEB2 expression in mouse brain. With reverse transcription polymerase chain reaction (RT-PCR) of total mouse brain cDNA, we identified four distinct CPEB2 splice variants. Single-cell RT-PCR showed that CPEB2 is predominantly expressed in neurons of the juvenile and adult brain and that individual cells express different sets of splice variants. Staining of brain slices with a custom-made CPEB2 antibody revealed ubiquitous expression of the protein in many brain regions, including hippocampus, striatum, thalamus, cortex, and cerebellum. We also found differential expression of CPEB2 protein in excitatory, inhibitory, and dopaminergic neurons. In primary hippocampal cultures, the subcellular localization of CPEB2 in neurons and astrocytes resembled that of CPEB1. Electrophoretic mobility shift assay and RNA coimmunoprecipitation revealed CPEB2 interaction with β-catenin and Ca(2+) /calmodulin-dependent protein kinase II (both established CPEB1 targets), indicating an overlap in RNA binding specificity between CPEB1 and CPEB2. Furthermore, we identified ephrin receptor A4 as a putative novel target of CPEB2. In conclusion, our study identifies CPEB2 splice variants to be differentially expressed among individual cells and across cell types of the mouse hippocampus, and reveals overlapping binding specificity between CPEB2 and CPEB1. 342 - Disease Mechanisms and Model Systems (POF3-342) G:(DE-HGF)POF3-342 POF3-342 POF III 0 2015-09-01 Crossref http://doi.wiley.com/10.1002/tdm_license_1.1 Dataset connected to CrossRef, PubMed, CPEB2 protein, mouse NLM Chemicals Cpeb1 protein, mouse NLM Chemicals Protein Isoforms NLM Chemicals RNA, Messenger NLM Chemicals RNA-Binding Proteins NLM Chemicals Transcription Factors NLM Chemicals beta Catenin NLM Chemicals mRNA Cleavage and Polyadenylation Factors NLM Chemicals RNA 63231-63-0 NLM Chemicals Receptor, EphA4 EC 2.7.10.1 NLM Chemicals Calcium-Calmodulin-Dependent Protein Kinase Type 2 EC 2.7.11.17 NLM Chemicals Animals MeSH Astrocytes: metabolism MeSH Brain: growth & development MeSH Brain: metabolism MeSH Calcium-Calmodulin-Dependent Protein Kinase Type 2: metabolism MeSH HeLa Cells MeSH Humans MeSH Mice MeSH Neurons: metabolism MeSH Protein Isoforms MeSH RNA: metabolism MeSH RNA, Messenger: metabolism MeSH RNA-Binding Proteins: genetics MeSH RNA-Binding Proteins: metabolism MeSH Receptor, EphA4: metabolism MeSH Transcription Factors: genetics MeSH Transcription Factors: metabolism MeSH Transfection MeSH beta Catenin: metabolism MeSH mRNA Cleavage and Polyadenylation Factors: genetics MeSH mRNA Cleavage and Polyadenylation Factors: metabolism MeSH Bedner, Peter 1 Skubal, Magdalena 2 Vangoor, Vamshidhar Reddy 3 Kaczmarczyk, Lech P:(DE-2719)2810486 4 dzne Karl, Kevin 5 Zoidl, Georg 6 Gieselmann, Volkmar 7 Seifert, Gerald 8 Steinhäuser, Christian 9 Kandel, Eric 10 Theis, Martin 11 10.1002/hipo.22399 : Wiley, 2014-12-23 5 630-642 journal-article Crossref Hippocampus 25 2014 1050-9631 10.1002/hipo.22399 Vol. 25, no. 5, p. 630 - 642 PERI:(DE-600)1498049-6 5 25:5<630 - 642 630-642 Hippocampus 25 2015 1050-9631 http://pub.dzne.de/record/141160/files/DZNE-2020-07482_Restricted.pdf http://pub.dzne.de/record/141160/files/DZNE-2020-07482_Restricted.pdf?subformat=pdfa pdfa VDB oai:pub.dzne.de:141160 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 4 P:(DE-2719)2810486 DE-HGF Gesundheit Erkrankungen des Nervensystems G:(DE-HGF)POF3-340 G:(DE-HGF)POF3-342 G:(DE-HGF)POF3 G:(DE-HGF)POF3-300 G:(DE-HGF)POF Disease Mechanisms and Model Systems 0 2015 Nationallizenz StatID:(DE-HGF)0420 StatID JCR StatID:(DE-HGF)0100 StatID HIPPOCAMPUS : 2017 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS DBCoverage StatID:(DE-HGF)0300 StatID Medline DBCoverage StatID:(DE-HGF)0310 StatID NCBI Molecular Biology Database DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List WoS StatID:(DE-HGF)0110 StatID Science Citation Index DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection WoS StatID:(DE-HGF)0111 StatID Science Citation Index Expanded DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - 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