153368 20240321221042.0 10.1038/s41590-020-0707-4 doi pmid:32541832 pmid 1529-2908 ISSN 1529-2916 ISSN altmetric:84050812 altmetric DZNE-2020-01365 English 610 Masuda, Takahiro P:(DE-HGF)0 0 Corresponding author Novel Hexb-based tools for studying microglia in the CNS. London 2020 Springer Nature Limited article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1704807496_29464 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Microglia and central nervous system (CNS)-associated macrophages (CAMs), such as perivascular and meningeal macrophages, are implicated in virtually all diseases of the CNS. However, little is known about their cell-type-specific roles in the absence of suitable tools that would allow for functional discrimination between the ontogenetically closely related microglia and CAMs. To develop a new microglia gene targeting model, we first applied massively parallel single-cell analyses to compare microglia and CAM signatures during homeostasis and disease and identified hexosaminidase subunit beta (Hexb) as a stably expressed microglia core gene, whereas other microglia core genes were substantially downregulated during pathologies. Next, we generated HexbtdTomato mice to stably monitor microglia behavior in vivo. Finally, the Hexb locus was employed for tamoxifen-inducible Cre-mediated gene manipulation in microglia and for fate mapping of microglia but not CAMs. In sum, we provide valuable new genetic tools to specifically study microglia functions in the CNS. 344 - Clinical and Health Care Research (POF3-344) G:(DE-HGF)POF3-344 POF3-344 POF III 0 Dataset connected to CrossRef, PubMed, Luminescent Agents NLM Chemicals Luminescent Proteins NLM Chemicals red fluorescent protein NLM Chemicals beta-Hexosaminidase beta Chain EC 3.2.1.52 NLM Chemicals Animals MeSH Brain: cytology MeSH Brain: immunology MeSH Brain: pathology MeSH CRISPR-Cas Systems: genetics MeSH Encephalomyelitis, Autoimmune, Experimental: immunology MeSH Encephalomyelitis, Autoimmune, Experimental: pathology MeSH Facial Nerve Injuries: immunology MeSH Facial Nerve Injuries: pathology MeSH Gene Knock-In Techniques MeSH Genes, Reporter: genetics MeSH Genetic Loci: genetics MeSH Humans MeSH Intravital Microscopy MeSH Luminescent Agents: chemistry MeSH Luminescent Proteins: chemistry MeSH Luminescent Proteins: genetics MeSH Macrophages: immunology MeSH Macrophages: metabolism MeSH Mice MeSH Microglia: immunology MeSH Microglia: metabolism MeSH NIH 3T3 Cells MeSH RNA-Seq MeSH Single-Cell Analysis MeSH Transfection MeSH beta-Hexosaminidase beta Chain: genetics MeSH beta-Hexosaminidase beta Chain: metabolism MeSH Amann, Lukas P:(DE-HGF)0 1 Sankowski, Roman P:(DE-HGF)0 2 Staszewski, Ori P:(DE-HGF)0 3 Lenz, Maximilian P:(DE-HGF)0 4 D Errico, Paolo P:(DE-HGF)0 5 Snaidero, Nicolas P:(DE-HGF)0 6 Costa Jordão, Marta Joana P:(DE-HGF)0 7 Böttcher, Chotima P:(DE-HGF)0 8 Kierdorf, Katrin P:(DE-HGF)0 9 Jung, Steffen P:(DE-HGF)0 10 Priller, Josef P:(DE-2719)2811122 11 dzne Misgeld, Thomas P:(DE-HGF)0 12 Vlachos, Andreas P:(DE-HGF)0 13 Meyer-Luehmann, Melanie P:(DE-HGF)0 14 Knobeloch, Klaus-Peter P:(DE-HGF)0 15 Prinz, Marco P:(DE-HGF)0 16 10.1038/s41590-020-0707-4 Vol. 21, no. 7, p. 802 - 815 PERI:(DE-600)2026412-4 7 802 - 815 Nature immunology 21 2020 1529-2916 http://pub.dzne.de/record/153368/files/DZNE-2020-01365.pdf http://pub.dzne.de/record/153368/files/DZNE-2020-01365.pdf?subformat=pdfa pdfa VDB oai:pub.dzne.de:153368 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 11 P:(DE-2719)2811122 DE-HGF Gesundheit Erkrankungen des Nervensystems G:(DE-HGF)POF3-340 G:(DE-HGF)POF3-344 G:(DE-HGF)POF3 G:(DE-HGF)POF3-300 G:(DE-HGF)POF Clinical and Health Care Research 0 2020 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2020-08-29 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2020-08-29 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2020-08-29 Nationallizenz StatID:(DE-HGF)0420 StatID 2023-03-30 ger JCR StatID:(DE-HGF)0100 StatID NAT IMMUNOL : 2021 2023-03-30 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2023-03-30 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2023-03-30 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2023-03-30 Peer Review StatID:(DE-HGF)0030 StatID ASC 2023-03-30 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2023-03-30 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2023-03-30 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2023-03-30 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2023-03-30 IF >= 30 StatID:(DE-HGF)9930 StatID NAT IMMUNOL : 2021 2023-03-30 I:(DE-2719)5000007 AG Priller Translational Neuropsychiatry 0 journal VDB I:(DE-2719)5000007 UNRESTRICTED