154669 20240411115843.0 10.1038/s41598-021-84887-2 doi pmid:33686166 pmid pmc:PMC7940647 pmc altmetric:102330191 altmetric DZNE-2021-00298 English 600 Kaczmarczyk, Lech P:(DE-2719)2810486 0 First author dzne Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background. [London] 2021 Macmillan Publishers Limited, part of Springer Nature article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1712750158_11351 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Genetic variation is a primary determinant of phenotypic diversity. In laboratory mice, genetic variation can be a serious experimental confounder, and thus minimized through inbreeding. However, generalizations of results obtained with inbred strains must be made with caution, especially when working with complex phenotypes and disease models. Here we compared behavioral characteristics of C57Bl/6-the strain most widely used in biomedical research-with those of 129S4. In contrast to 129S4, C57Bl/6 demonstrated high within-strain and intra-litter behavioral hyperactivity. Although high consistency would be advantageous, the majority of disease models and transgenic tools are in C57Bl/6. We recently established six Cre driver lines and two Cre effector lines in 129S4. To augment this collection, we genetically engineered a Cre line to study astrocytes in 129S4. It was validated with two Cre effector lines: calcium indicator gCaMP5g-tdTomato and RiboTag-a tool widely used to study cell type-specific translatomes. These reporters are in different genomic loci, and in both the Cre was functional and astrocyte-specific. We found that calcium signals lasted longer and had a higher amplitude in cortical compared to hippocampal astrocytes, genes linked to a single neurodegenerative disease have highly divergent expression patterns, and that ribosome proteins are non-uniformly expressed across brain regions and cell types. 353 - Clinical and Health Care Research (POF4-353) G:(DE-HGF)POF4-353 POF4-353 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Animals MeSH Excitatory Amino Acid Transporter 1: genetics MeSH Excitatory Amino Acid Transporter 1: metabolism MeSH Integrases MeSH Mice MeSH Mice, Transgenic MeSH Neurodegenerative Diseases: genetics MeSH Neurodegenerative Diseases: metabolism MeSH Neuroglia: metabolism MeSH Reichenbach, Nicole P:(DE-2719)2811281 1 dzne Blank, Nelli P:(DE-2719)2811722 2 dzne Jonson, Maria P:(DE-HGF)0 3 Dittrich, Lars P:(DE-2719)2811120 4 dzne Petzold, Gabor C P:(DE-2719)2810273 5 dzne Jackson, Walker S P:(DE-2719)2810253 6 Last author dzne 10.1038/s41598-021-84887-2 Vol. 11, no. 1, p. 5412 PERI:(DE-600)2615211-3 1 5412 Scientific reports 11 2021 2045-2322 OpenAccess http://pub.dzne.de/record/154669/files/DZNE-2021-00298.pdf OpenAccess pdfa http://pub.dzne.de/record/154669/files/DZNE-2021-00298.pdf?subformat=pdfa oai:pub.dzne.de:154669 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)2810486 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)2811281 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)2811722 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 4 P:(DE-2719)2811120 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 5 P:(DE-2719)2810273 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)2810253 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-353 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Clinical and Health Care Research 0 DE-HGF Gesundheit Erkrankungen des Nervensystems G:(DE-HGF)POF3-340 G:(DE-HGF)POF3-344 G:(DE-HGF)POF3 G:(DE-HGF)POF3-300 G:(DE-HGF)POF Clinical and Health Care Research 0 2021 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2023-03-30 Creative Commons Attribution CC BY (No Version) LIC:(DE-HGF)CCBYNV V:(DE-HGF) DOAJ 2021-02-03 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2023-03-30 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2021-02-03 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2023-03-30 DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2023-03-30 JCR StatID:(DE-HGF)0100 StatID SCI REP-UK : 2021 2023-03-30 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2022-08-08T09:38:07Z WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2021-02-03 DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2022-08-08T09:38:07Z DBCoverage StatID:(DE-HGF)0300 StatID Medline 2023-03-30 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2023-03-30 IF < 5 StatID:(DE-HGF)9900 StatID 2023-03-30 OpenAccess StatID:(DE-HGF)0510 StatID Peer Review StatID:(DE-HGF)0030 StatID ASC 2023-03-30 Article Processing Charges StatID:(DE-HGF)0561 StatID 2021-02-03 DBCoverage StatID:(DE-HGF)1150 StatID Current Contents - Physical, Chemical and Earth Sciences 2023-03-30 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2021-02-03 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2023-03-30 Fees StatID:(DE-HGF)0700 StatID 2021-02-03 yes I:(DE-2719)1013020 AG Petzold ; AG Petzold Vascular Neurology 0 I:(DE-2719)1013019 AG Jackson Selective Vulnerability of Neurodegenerative Diseases 1 journal VDB UNRESTRICTED I:(DE-2719)1013020 I:(DE-2719)1013019 FullTexts