163742 20230915090534.0 10.1371/journal.pbio.3001503 doi pmid:35312684 pmid pmc:PMC8970474 pmc 1544-9173 ISSN 1545-7885 ISSN altmetric:125080596 altmetric DZNE-2022-00481 English 610 Schmerl, Bettina 0 The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. Lawrence, KS 2022 PLoS article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1655120870_6957 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Recent advances in imaging technology have highlighted that scaffold proteins and receptors are arranged in subsynaptic nanodomains. The synaptic membrane-associated guanylate kinase (MAGUK) scaffold protein membrane protein palmitoylated 2 (MPP2) is a component of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-associated protein complexes and also binds to the synaptic cell adhesion molecule SynCAM 1. Using superresolution imaging, we show that-like SynCAM 1-MPP2 is situated at the periphery of the postsynaptic density (PSD). In order to explore MPP2-associated protein complexes, we used a quantitative comparative proteomics approach and identified multiple γ-aminobutyric acid (GABA)A receptor subunits among novel synaptic MPP2 interactors. In line with a scaffold function for MPP2 in the assembly and/or modulation of intact GABAA receptors, manipulating MPP2 expression had effects on inhibitory synaptic transmission. We further show that GABAA receptors are found together with MPP2 in a subset of dendritic spines and thus highlight MPP2 as a scaffold that serves as an adaptor molecule, linking peripheral synaptic elements critical for inhibitory regulation to central structures at the PSD of glutamatergic synapses. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Membrane Proteins NLM Chemicals Receptors, AMPA NLM Chemicals Receptors, GABA-A NLM Chemicals Membrane Proteins: metabolism MeSH Post-Synaptic Density: metabolism MeSH Receptors, AMPA: metabolism MeSH Receptors, GABA-A MeSH Synapses: metabolism MeSH Gimber, Niclas 1 Kuropka, Benno 2 Stumpf, Alexander 3 Rentsch, Jakob 4 Kunde, Stella-Amrei 5 von Sivers, Judith 6 Ewers, Helge 7 Schmitz, Dietmar P:(DE-2719)2810725 8 dzne Freund, Christian 9 Schmoranzer, Jan 10 Rademacher, Nils P:(DE-2719)2814334 11 dzne Shoichet, Sarah A 0000-0003-4933-7846 12 10.1371/journal.pbio.3001503 Vol. 20, no. 3, p. e3001503 - PERI:(DE-600)2126773-X 3 e3001503 PLoS biology 20 2022 1544-9173 OpenAccess http://pub.dzne.de/record/163742/files/DZNE-2022-00481.pdf OpenAccess pdfa http://pub.dzne.de/record/163742/files/DZNE-2022-00481.pdf?subformat=pdfa oai:pub.dzne.de:163742 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 8 P:(DE-2719)2810725 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 11 P:(DE-2719)2814334 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2022 Article Processing Charges StatID:(DE-HGF)0561 StatID 2021-01-26 Creative Commons Attribution CC BY (No Version) LIC:(DE-HGF)CCBYNV V:(DE-HGF) DOAJ 2021-01-26 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2021-01-26 Fees StatID:(DE-HGF)0700 StatID 2021-01-26 OpenAccess StatID:(DE-HGF)0510 StatID DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2021-01-26 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2021-01-26 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2022-11-11 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2022-11-11 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2022-01-10T10:28:08Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2022-01-10T10:28:08Z Peer Review StatID:(DE-HGF)0030 StatID DOAJ : Blind peer review 2022-01-10T10:28:08Z DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2022-11-11 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2022-11-11 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2022-11-11 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2022-11-11 DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2022-11-11 DBCoverage StatID:(DE-HGF)1060 StatID Current Contents - Agriculture, Biology and Environmental Sciences 2022-11-11 JCR StatID:(DE-HGF)0100 StatID PLOS BIOL : 2021 2022-11-11 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2022-11-11 Peer Review StatID:(DE-HGF)0030 StatID ASC 2022-11-11 IF >= 5 StatID:(DE-HGF)9905 StatID PLOS BIOL : 2021 2022-11-11 I:(DE-2719)1810004 AG Schmitz 1 Network Dysfunction 0 I:(DE-2719)1810001 AG Garner Synaptopathy 1 journal VDB UNRESTRICTED I:(DE-2719)1830000 I:(DE-2719)1810001 FullTexts