163763 20240319115431.0 pmc:PMC9543601 pmc 10.1111/febs.16425 doi pmid:35263507 pmid 0014-2956 ISSN 0945-5795 ISSN 1432-1033 ISSN 1742-464X ISSN 1742-4658 ISSN altmetric:124380129 altmetric DZNE-2022-00502 English 610 Aalto, Anna 0 M1-linked ubiquitination facilitates NF-κB activation and survival during sterile inflammation. Oxford [u.a.] 2022 Wiley-Blackwell article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1665148364_1933 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote CC BY-NC-ND: https://creativecommons.org/licenses/by-nc-nd/4.0/ Methionine 1 (M1)-linked ubiquitination plays a key role in the regulation of inflammatory nuclear factor-κB (NF-κB) signalling and is important for clearance of pathogen infection in Drosophila melanogaster. M1-linked ubiquitin (M1-Ub) chains are assembled by the linear ubiquitin E3 ligase (LUBEL) in flies. Here, we have studied the role of LUBEL in sterile inflammation induced by different types of cellular stresses. We have found that the LUBEL catalyses formation of M1-Ub chains in response to hypoxic, oxidative and mechanical stress conditions. LUBEL is shown to be important for flies to survive low oxygen conditions and paraquat-induced oxidative stress. This protective action seems to be driven by stress-induced activation of the NF-κB transcription factor Relish via the immune deficiency (Imd) pathway. In addition to LUBEL, the intracellular mediators of Relish activation, including the transforming growth factor activating kinase 1 (Tak1), Drosophila inhibitor of apoptosis (IAP) Diap2, the IκB kinase γ (IKKγ) Kenny and the initiator caspase Death-related ced-3/Nedd2-like protein (Dredd), but not the membrane receptor peptidoglycan recognition protein (PGRP)-LC, are shown to be required for sterile inflammatory response and survival. Finally, we showed that the stress-induced upregulation of M1-Ub chains in response to hypoxia, oxidative and mechanical stress is also induced in mammalian cells and protects from stress-induced cell death. Taken together, our results suggest that M1-Ub chains are important for NF-κB signalling in inflammation induced by stress conditions often observed in chronic inflammatory diseases and cancer. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de NF-κB Other cell stress Other hypoxia Other linear ubiquitin chain Other sterile inflammation Other Animals MeSH Drosophila: metabolism MeSH Drosophila Proteins: genetics MeSH Drosophila Proteins: metabolism MeSH Drosophila melanogaster: metabolism MeSH Inflammation: genetics MeSH MAP Kinase Kinase Kinases: metabolism MeSH Mammals: metabolism MeSH Methionine: metabolism MeSH NF-kappa B: genetics MeSH NF-kappa B: metabolism MeSH Ubiquitin: metabolism MeSH Ubiquitination MeSH Martínez-Chacón, Gabriela 1 Kietz, Christa 2 Tsyganova, Nadezhda 3 Kreutzer, Joose 4 Kallio, Pasi 5 Broemer, Meike P:(DE-2719)2810307 6 dzne Meinander, Annika 0000-0002-3878-2293 7 10.1111/febs.16425 p. febs.16425 PERI:(DE-600)2172518-4 17 5180-5197 The FEBS journal 289 2022 0014-2956 OpenAccess http://pub.dzne.de/record/163763/files/DZNE-2022-00502.pdf OpenAccess pdfa http://pub.dzne.de/record/163763/files/DZNE-2022-00502.pdf?subformat=pdfa oai:pub.dzne.de:163763 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)2810307 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2022 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2022-11-12 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2022-11-12 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2022-11-12 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2021-01-30 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2022-11-12 Creative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0 LIC:(DE-HGF)CCBYNCND4 HGFVOC JCR StatID:(DE-HGF)0100 StatID FEBS J : 2021 2022-11-12 DEAL Wiley StatID:(DE-HGF)3001 StatID 2021-01-30 ger WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2021-01-30 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2022-11-12 OpenAccess StatID:(DE-HGF)0510 StatID DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2022-11-12 Peer Review StatID:(DE-HGF)0030 StatID ASC 2022-11-12 IF >= 5 StatID:(DE-HGF)9905 StatID FEBS J : 2021 2022-11-12 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2021-01-30 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2022-11-12 I:(DE-2719)1013021 AG Brömer 2 AG Brömer 0 journal VDB UNRESTRICTED I:(DE-2719)1013021 FullTexts