258922 20231120155350.0 10.3390/life13061254 doi pmid:37374039 pmid pmc:PMC10303776 pmc altmetric:148947330 altmetric DZNE-2023-00694 English 570 Buchner, Felix P:(DE-2719)9001904 0 First author dzne Spinal Cord Organoids to Study Motor Neuron Development and Disease. Basel 2023 MDPI article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1688649352_17439 PUB:(DE-HGF) Review Article ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Motor neuron diseases (MNDs) are a heterogeneous group of disorders that affect the cranial and/or spinal motor neurons (spMNs), spinal sensory neurons and the muscular system. Although they have been investigated for decades, we still lack a comprehensive understanding of the underlying molecular mechanisms; and therefore, efficacious therapies are scarce. Model organisms and relatively simple two-dimensional cell culture systems have been instrumental in our current knowledge of neuromuscular disease pathology; however, in the recent years, human 3D in vitro models have transformed the disease-modeling landscape. While cerebral organoids have been pursued the most, interest in spinal cord organoids (SCOs) is now also increasing. Pluripotent stem cell (PSC)-based protocols to generate SpC-like structures, sometimes including the adjacent mesoderm and derived skeletal muscle, are constantly being refined and applied to study early human neuromuscular development and disease. In this review, we outline the evolution of human PSC-derived models for generating spMN and recapitulating SpC development. We also discuss how these models have been applied to exploring the basis of human neurodevelopmental and neurodegenerative diseases. Finally, we provide an overview of the main challenges to overcome in order to generate more physiologically relevant human SpC models and propose some exciting new perspectives. 352 - Disease Mechanisms (POF4-352) G:(DE-HGF)POF4-352 POF4-352 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de development Other in vitro disease modeling Other induced pluripotent stem cells (iPSCs) Other motor neuron (MN) Other motor neuron diseases (MNDs) Other organoids Other spinal cord (SpC) Other spinal cord organoids (SCOs) Other Dokuzluoglu, Zeynep P:(DE-2719)9002364 1 dzne Grass, Tobias P:(DE-2719)9000564 2 dzne Rodriguez-Muela, Natalia P:(DE-2719)9000726 3 Last author dzne Molecular and Cellular Mechanisms in Neurodegenerative Diseases 10.3390/life13061254 Vol. 13, no. 6, p. 1254 - PERI:(DE-600)2662250-6 6 1254 Life 13 2023 2075-1729 https://www.mdpi.com/2075-1729/13/6/1254 http://pub.dzne.de/record/258922/files/DZNE-2023-00694.pdf OpenAccess http://pub.dzne.de/record/258922/files/DZNE-2023-00694.pdf?subformat=pdfa pdfa OpenAccess oai:pub.dzne.de:258922 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)9001904 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)9002364 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)9000564 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)9000726 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-352 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Disease Mechanisms 0 2023 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2023-03-30 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2023-03-30 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2023-03-30 Fees StatID:(DE-HGF)0700 StatID 2023-03-30 OpenAccess StatID:(DE-HGF)0510 StatID Article Processing Charges StatID:(DE-HGF)0561 StatID 2023-03-30 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2023-04-12T15:01:09Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2023-04-12T15:01:09Z Peer Review StatID:(DE-HGF)0030 StatID DOAJ : Anonymous peer review 2023-04-12T15:01:09Z JCR StatID:(DE-HGF)0100 StatID LIFE-BASEL : 2022 2023-10-26 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2023-10-26 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2023-10-26 DBCoverage StatID:(DE-HGF)0320 StatID PubMed Central 2023-10-26 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2023-10-26 Peer Review StatID:(DE-HGF)0030 StatID ASC 2023-10-26 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2023-10-26 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2023-10-26 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2023-10-26 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2023-10-26 IF < 5 StatID:(DE-HGF)9900 StatID 2023-10-26 I:(DE-2719)1713001 AG Rodriguez-Muela Selective Neuronal Vulnerability in Neurodegenerative Diseases 0 journal VDB UNRESTRICTED I:(DE-2719)1713001 FullTexts