263802 20231120155355.0 10.15252/embr.202256702 doi pmid:37477166 pmid pmc:PMC10481675 pmc 1469-221X ISSN 1469-3178 ISSN DZNE-2023-00888 English 570 Michanski, Susann 0 Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing. Hoboken, NJ [u.a.] 2023 Wiley article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1695288736_8402 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Cochlear inner hair cells (IHCs) form specialized ribbon synapses with spiral ganglion neurons that tirelessly transmit sound information at high rates over long time periods with extreme temporal precision. This functional specialization is essential for sound encoding and is attributed to a distinct molecular machinery with unique players or splice variants compared to conventional neuronal synapses. Among these is the active zone (AZ) scaffold protein piccolo/aczonin, which is represented by its short splice variant piccolino at cochlear and retinal ribbon synapses. While the function of piccolo at synapses of the central nervous system has been intensively investigated, the role of piccolino at IHC synapses remains unclear. In this study, we characterize the structure and function of IHC synapses in piccolo gene-trap mutant rats (Pclogt/gt ). We find a mild hearing deficit with elevated thresholds and reduced amplitudes of auditory brainstem responses. Ca2+ channel distribution and ribbon morphology are altered in apical IHCs, while their presynaptic function seems to be unchanged. We conclude that piccolino contributes to the AZ organization in IHCs and is essential for normal hearing. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Ca2+ channel Other active zone Other cochlea Other hearing Other synaptic vesicle Other Pclo protein, rat NLM Chemicals Cytoskeletal Proteins NLM Chemicals Neuropeptides NLM Chemicals Rats MeSH Animals MeSH Hair Cells, Auditory, Inner MeSH Hearing: physiology MeSH Synapses: physiology MeSH Cochlea MeSH Spiral Ganglion: metabolism MeSH Cytoskeletal Proteins: metabolism MeSH Neuropeptides MeSH Kapoor, Rohan 0000-0002-4522-3289 1 Steyer, Anna M 0000-0002-4814-7517 2 Möbius, Wiebke 3 Früholz, Iris 4 Ackermann, Frauke P:(DE-2719)2810967 5 dzne Gültas, Mehmet 6 Garner, Craig C P:(DE-2719)2810922 7 dzne Hamra, F Kent 8 Neef, Jakob 0000-0002-4757-9385 9 Strenzke, Nicola 0000-0003-1673-1046 10 Moser, Tobias 0000-0001-7145-0533 11 Wichmann, Carolin 0000-0001-8868-8716 12 10.15252/embr.202256702 Vol. 24, no. 9, p. e56702 PERI:(DE-600)2025376-X 9 e56702 EMBO reports 24 2023 1469-221X http://pub.dzne.de/record/263802/files/DZNE-2023-00888_Restricted.pdf http://pub.dzne.de/record/263802/files/DZNE-2023-00888_Restricted.pdf?subformat=pdfa pdfa oai:pub.dzne.de:263802 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 5 P:(DE-2719)2810967 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 7 P:(DE-2719)2810922 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2023 JCR StatID:(DE-HGF)0100 StatID EMBO REP : 2022 2023-10-24 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2023-10-24 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2023-10-24 DBCoverage StatID:(DE-HGF)0320 StatID PubMed Central 2023-10-24 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2023-10-24 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2023-10-24 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2023-10-24 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2023-10-24 IF >= 5 StatID:(DE-HGF)9905 StatID EMBO REP : 2022 2023-10-24 I:(DE-2719)1813004 AG Ackermann Astrocyte - Synapse Interactions 0 I:(DE-2719)1810001 AG Garner Synaptopathy 1 journal VDB I:(DE-2719)1813004 I:(DE-2719)1810001 UNRESTRICTED