276154 20260105141603.0 10.1016/j.nbd.2025.106802 doi pmid:39800229 pmid 0969-9961 ISSN 1095-953X ISSN altmetric:173120170 altmetric DZNE-2025-00226 English 570 Arshad, Hamza 0 The brain interactome of a permissive prion replication substrate. [Amsterdam] 2025 Elsevier article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1738668993_26729 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Bank voles are susceptible to prion strains from many different species, yet the molecular mechanisms underlying the ability of bank vole prion protein (BVPrP) to function as a universal prion acceptor remain unclear. Potential differences in molecular environments and protein interaction networks on the cell surface of brain cells may contribute to BVPrP's unusual behavior. To test this hypothesis, we generated knock-in mice that express physiological levels of BVPrP (M109 isoform) and employed mass spectrometry to compare the interactomes of mouse (Mo) PrP and BVPrP following mild in vivo crosslinking of brain tissue. Substantial overlap was observed between the top interactors for BVPrP and MoPrP, with established PrP-interactors such as neural cell adhesion molecules, subunits of Na+/K+-ATPases, and contactin-1 being equally present in the two interactomes. We conclude that the molecular environments of BVPrP and MoPrP in the brains of mice are very similar. This suggests that the unorthodox properties of BVPrP are unlikely to be mediated by differential interactions with other proteins. 899 - ohne Topic (POF4-899) G:(DE-HGF)POF4-899 POF4-899 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Bank voles Other Knock-in mice Other Mass spectrometry Other Prion Other Protein-protein interactions Other Animals MeSH Brain: metabolism MeSH Mice MeSH Arvicolinae MeSH Prions: metabolism MeSH Prions: genetics MeSH Gene Knock-In Techniques MeSH Mice, Transgenic MeSH Mice, Inbred C57BL MeSH Eid, Shehab 1 Mehra, Surabhi 2 Williams, Declan 3 Kaczmarczyk, Lech P:(DE-2719)2810486 4 Stuart, Erica 5 Jackson, Walker Scot P:(DE-2719)2810253 6 Schmitt-Ulms, Gerold 7 Watts, Joel C 8 10.1016/j.nbd.2025.106802 Vol. 206, p. 106802 - PERI:(DE-600)1471408-5 106802 Neurobiology of disease 206 2025 0969-9961 http://pub.dzne.de/record/276154/files/DZNE-2025-00226%20SUP.zip OpenAccess http://pub.dzne.de/record/276154/files/DZNE-2025-00226.pdf OpenAccess pdfa http://pub.dzne.de/record/276154/files/DZNE-2025-00226.pdf?subformat=pdfa oai:pub.dzne.de:276154 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 4 P:(DE-2719)2810486 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)2810253 DE-HGF Programmungebundene Forschung ohne Programm G:(DE-HGF)POF4-890 G:(DE-HGF)POF4-899 G:(DE-HGF)POF4 G:(DE-HGF)POF4-800 G:(DE-HGF)POF ohne Topic 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2025-01-01 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2025-01-01 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2025-01-01 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2025-01-01 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2025-01-01 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2025-01-01 IF >= 5 StatID:(DE-HGF)9905 StatID NEUROBIOL DIS : 2022 2025-01-01 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2023-04-12T14:49:08Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2023-04-12T14:49:08Z DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2025-01-01 Fees StatID:(DE-HGF)0700 StatID 2025-01-01 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2025-01-01 OpenAccess StatID:(DE-HGF)0510 StatID Peer Review StatID:(DE-HGF)0030 StatID ASC 2025-01-01 Article Processing Charges StatID:(DE-HGF)0561 StatID 2025-01-01 JCR StatID:(DE-HGF)0100 StatID NEUROBIOL DIS : 2022 2025-01-01 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2025-01-01 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2025-01-01 I:(DE-2719)1013019 AG Jackson Selective Vulnerability of Neurodegenerative Diseases 0 journal VDB UNRESTRICTED I:(DE-2719)1013019 FullTexts