276280 20260105141714.0 10.1007/s12035-024-04365-4 doi pmid:39012444 pmid 0893-7648 ISSN 1559-1182 ISSN DZNE-2025-00259 English 570 Owona, Brice Ayissi 0000-0002-2380-527X 0 First author Biflavonoid Methylchamaejasmin and Khaya grandifoliola Extract Inhibit NLRP3 Inflammasome in THP-1 Cell Model of Neuroinflammation. Totowa, NJ 2025 Humana Press article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1738671434_26729 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Neuroinflammation is a common hallmark of Alzheimer's disease (AD), with NLRP3 inflammasome proven to be activated in microglia of AD patients' brains. In this study, a newly isolated biflavonoid (7,7'-di-O-methylchamaejasmin/M8) and a crude extract of the plant Khaya grandifoliola (KG) were investigated for their inhibitory effect on inflammasome activation. In preliminary experiments, M8 and KG showed no cytotoxicity on human macrophage-like differentiated THP-1 cells and exhibited anti-inflammatory inhibition of nitric oxide produced following lipopolysaccharide stimulation. Furthermore, M8 and KG blocked IL-1β and IL-18 production by reducing NLRP3 inflammasome components including NFκB, NLRP3, Caspase-1, pro-IL-1β, and pro-IL-18 at the mRNA and protein levels. Regarding the formation of ASC (apoptosis-associated speck-like protein containing a CARD) specks during inflammasome activation, the size and fluorescent intensity of the existing specks were unchanged across all treatment conditions. However, M8 and KG treatments were shown to prevent further speck formation. In addition, experiments on amyloid β phagocytosis showed that M8 and KG pretreatments can restore the phagocytic activity of THP-1 cells, which was impaired following inflammasome activation. Altogether, our findings describe for the first time a promising role of biflavonoids and KG extract in preventing inflammasome activation and protecting against neuroinflammation, a key factor in AD development. 353 - Clinical and Health Care Research (POF4-353) G:(DE-HGF)POF4-353 POF4-353 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Khaya grandifoliola Other 7,7′-di-O-Methylchamaejasmin Other Biflavonoid Other NLRP3 inflammasome Other THP-1 cells Other NLR Family, Pyrin Domain-Containing 3 Protein NLM Chemicals Inflammasomes NLM Chemicals Plant Extracts NLM Chemicals Biflavonoids NLM Chemicals Amyloid beta-Peptides NLM Chemicals Lipopolysaccharides NLM Chemicals Nitric Oxide 31C4KY9ESH NLM Chemicals Interleukin-1beta NLM Chemicals NLR Family, Pyrin Domain-Containing 3 Protein: antagonists & inhibitors MeSH Humans MeSH NLR Family, Pyrin Domain-Containing 3 Protein: metabolism MeSH Inflammasomes: metabolism MeSH Inflammasomes: drug effects MeSH Plant Extracts: pharmacology MeSH THP-1 Cells MeSH Biflavonoids: pharmacology MeSH Biflavonoids: therapeutic use MeSH Neuroinflammatory Diseases: drug therapy MeSH Neuroinflammatory Diseases: metabolism MeSH Neuroinflammatory Diseases: pathology MeSH Amyloid beta-Peptides: metabolism MeSH Lipopolysaccharides: pharmacology MeSH Meliaceae: chemistry MeSH Phagocytosis: drug effects MeSH Nitric Oxide: metabolism MeSH Interleukin-1beta: metabolism MeSH Plant Extracts: therapeutic use MeSH Models, Biological MeSH Meliaceae MeSH Mary, Arnaud 0000-0002-6597-766X 1 Messi, Angelique N 0000-0002-9184-2080 2 Ravichandran, Kishore Aravind P:(DE-2719)9002244 3 Mbing, Josephine Ngo 0000-0002-1427-979X 4 Pegnyemb, Emmanuel 0000-0001-5431-621X 5 Moundipa, Paul F 0000-0002-2694-5569 6 Heneka, Michael P:(DE-2719)2000008 7 Last author 10.1007/s12035-024-04365-4 Vol. 62, no. 2, p. 1605 - 1619 PERI:(DE-600)2079384-4 2 1605 - 1619 Molecular neurobiology 62 2025 0893-7648 http://pub.dzne.de/record/276280/files/DZNE-2025-00259_Restricted.pdf http://pub.dzne.de/record/276280/files/DZNE-2025-00259_Restricted.pdf?subformat=pdfa pdfa oai:pub.dzne.de:276280 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 0000-0002-2380-527X Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)9002244 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 7 P:(DE-2719)2000008 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-353 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Clinical and Health Care Research 0 2025 DEAL Springer StatID:(DE-HGF)3002 StatID 2025-01-07 ger DEAL Springer StatID:(DE-HGF)3002 StatID 2025-01-07 ger DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2025-01-07 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2025-01-07 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2025-01-07 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2025-01-07 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2025-01-07 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2025-01-07 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2025-01-07 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2025-01-07 JCR StatID:(DE-HGF)0100 StatID MOL NEUROBIOL : 2022 2025-01-07 IF >= 5 StatID:(DE-HGF)9905 StatID MOL NEUROBIOL : 2022 2025-01-07 I:(DE-2719)1011303 AG Heneka Neuroinflammation, Biomarker 0 journal VDB I:(DE-2719)1011303 UNRESTRICTED