277728 20260105131112.0 10.1038/s41467-025-57985-2 doi pmid:40113768 pmid pmc:PMC11926106 pmc altmetric:175368751 altmetric DZNE-2025-00449 English 500 Mangiarotti, Agustin P:(DE-2719)9003374 0 First author dzne Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates. [London] 2025 Springer Nature article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1743593563_4325 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Biomolecular condensates play a central role in cellular processes by interacting with membranes driving wetting transitions and inducing mutual remodeling. While condensates are known to locally alter membrane properties such as lipid packing and hydration, it remains unclear how membrane composition and phase state in turn affect condensate affinity. Here, we show that it is not only the membrane phase itself, but rather the degree of lipid packing that determines the condensate affinity for membranes. Increasing lipid chain length, saturation, or cholesterol content, enhances lipid packing, thereby decreasing condensate interaction. This regulatory mechanism is consistent across various condensate-membrane systems, highlighting the critical role of the membrane interface. In addition, protein adsorption promotes extensive membrane remodeling, including the formation of tubes and double-membrane sheets. Our findings reveal a mechanism by which membrane composition fine-tunes condensate wetting, highlighting its potential impact on cellular functions and organelle interactions. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Cholesterol 97C5T2UQ7J NLM Chemicals Membrane Lipids NLM Chemicals Lipid Bilayers NLM Chemicals Lipids NLM Chemicals Cell Membrane: chemistry MeSH Cholesterol: metabolism MeSH Cholesterol: chemistry MeSH Wettability MeSH Biomolecular Condensates: metabolism MeSH Biomolecular Condensates: chemistry MeSH Cell Membrane: metabolism MeSH Membrane Lipids: metabolism MeSH Membrane Lipids: chemistry MeSH Lipid Bilayers: metabolism MeSH Lipid Bilayers: chemistry MeSH Lipids: chemistry MeSH Adsorption MeSH Sabri, Elias 1 Schmidt, Kita Valerie 2 Hoffmann, Christian P:(DE-2719)9000582 3 Milovanovic, Dragomir P:(DE-2719)9000670 4 Lipowsky, Reinhard 5 Dimova, Rumiana 0000-0002-3872-8502 6 10.1038/s41467-025-57985-2 Vol. 16, no. 1, p. 2756 PERI:(DE-600)2553671-0 1 2756 Nature Communications 16 2025 2041-1723 http://pub.dzne.de/record/277728/files/DZNE-2025-00449%20SUP%2BSRC.zip OpenAccess http://pub.dzne.de/record/277728/files/DZNE-2025-00449.pdf OpenAccess pdfa http://pub.dzne.de/record/277728/files/DZNE-2025-00449.pdf?subformat=pdfa oai:pub.dzne.de:277728 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)9003374 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)9000582 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 4 P:(DE-2719)9000670 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2025-01-02 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2025-01-02 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2025-01-02 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2025-01-02 OpenAccess StatID:(DE-HGF)0510 StatID DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2025-01-02 IF >= 15 StatID:(DE-HGF)9915 StatID NAT COMMUN : 2022 2025-01-02 JCR StatID:(DE-HGF)0100 StatID NAT COMMUN : 2022 2025-01-02 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2024-01-30T07:48:07Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2024-01-30T07:48:07Z DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2025-01-02 Fees StatID:(DE-HGF)0700 StatID 2025-01-02 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2025-01-02 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2025-01-02 Peer Review StatID:(DE-HGF)0030 StatID DOAJ : Peer review 2024-01-30T07:48:07Z Article Processing Charges StatID:(DE-HGF)0561 StatID 2025-01-02 DBCoverage StatID:(DE-HGF)1060 StatID Current Contents - Agriculture, Biology and Environmental Sciences 2025-01-02 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2025-01-02 DBCoverage StatID:(DE-HGF)1150 StatID Current Contents - Physical, Chemical and Earth Sciences 2025-01-02 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2025-01-02 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 I:(DE-2719)1013043 AG Milovanovic (Bonn) Molecular Neuroscience 1 journal VDB UNRESTRICTED I:(DE-2719)1813002 I:(DE-2719)1013043 FullTexts