278034 20250504001211.0 10.1126/sciadv.ads7627 doi pmid:40249817 pmid pmc:PMC12007584 pmc altmetric:176249485 altmetric DZNE-2025-00540 English 500 Wang, Huan P:(DE-HGF)0 0 Live-cell quantification reveals viscoelastic regulation of synapsin condensates by α-synuclein. Washington, DC [u.a.] 2025 Assoc. article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1745308888_8827 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Synapsin and α-synuclein represent a growing list of condensate-forming proteins where the material states of condensates are directly linked to cellular functions (e.g., neurotransmission) and pathology (e.g., neurodegeneration). However, quantifying condensate material properties in living systems has been a substantial challenge. Here, we develop micropipette aspiration and whole-cell patch-clamp (MAPAC), a platform that allows direct material quantification of condensates in live cells. We find 10,000-fold variations in the viscoelasticity of synapsin condensates, regulated by the partitioning of α-synuclein, a marker for synucleinopathies. Through in vitro reconstitutions, we identify multiple molecular factors that distinctly regulate the viscosity, interfacial tension, and maturation of synapsin condensates, confirming the cellular roles of α-synuclein. Overall, our study provides unprecedented quantitative insights into the material properties of neuronal condensates and reveals a crucial role of α-synuclein in regulating condensate viscoelasticity. Furthermore, we envision MAPAC applicable to study a broad range of condensates in vivo. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de alpha-Synuclein NLM Chemicals Synapsins NLM Chemicals alpha-Synuclein: metabolism MeSH alpha-Synuclein: chemistry MeSH Viscosity MeSH Humans MeSH Synapsins: metabolism MeSH Synapsins: chemistry MeSH Elasticity MeSH Animals MeSH Biomolecular Condensates: metabolism MeSH Biomolecular Condensates: chemistry MeSH Patch-Clamp Techniques MeSH Hoffmann, Christian P:(DE-2719)9000582 1 Tromm, Johannes V P:(DE-2719)9002092 2 dzne Su, Xiao 0000-0001-6408-8273 3 Elliott, Jordan 0009-0002-9175-4440 4 Wang, Han P:(DE-2719)9001572 5 dzne Deng, Mengying 0000-0003-3932-2552 6 McClenaghan, Conor 0000-0002-0859-3854 7 Baum, Jean 8 Pang, Zhiping P 0000-0002-6183-1233 9 Milovanovic, Dragomir P:(DE-2719)9000670 10 Shi, Zheng 0000-0002-5214-1871 11 10.1126/sciadv.ads7627 Vol. 11, no. 16, p. eads7627 PERI:(DE-600)2810933-8 16 eads7627 Science advances 11 2025 2375-2548 OpenAccess http://pub.dzne.de/record/278034/files/DZNE-2025-00540.pdf OpenAccess pdfa http://pub.dzne.de/record/278034/files/DZNE-2025-00540.pdf?subformat=pdfa oai:pub.dzne.de:278034 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)9000582 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)9002092 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 5 P:(DE-2719)9001572 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 10 P:(DE-2719)9000670 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2024-12-18 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2024-12-18 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2024-12-18 DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2024-12-18 JCR StatID:(DE-HGF)0100 StatID SCI ADV : 2022 2024-12-18 IF >= 10 StatID:(DE-HGF)9910 StatID SCI ADV : 2022 2024-12-18 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2024-05-14T07:33:42Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2024-05-14T07:33:42Z Creative Commons Attribution-NonCommercial CC BY-NC 4.0 LIC:(DE-HGF)CCBYNC4 HGFVOC WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2024-12-18 Fees StatID:(DE-HGF)0700 StatID 2024-12-18 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2024-12-18 OpenAccess StatID:(DE-HGF)0510 StatID Peer Review StatID:(DE-HGF)0030 StatID ASC 2024-12-18 Article Processing Charges StatID:(DE-HGF)0561 StatID 2024-12-18 DBCoverage StatID:(DE-HGF)1150 StatID Current Contents - Physical, Chemical and Earth Sciences 2024-12-18 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2024-12-18 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2024-12-18 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 I:(DE-2719)1013043 AG Milovanovic (Bonn) Molecular Neuroscience 1 journal VDB UNRESTRICTED I:(DE-2719)1813002 I:(DE-2719)1013043 FullTexts