280243 20250827101637.0 978-1-0716-4681-6 (print) 978-1-0716-4682-3 (electronic) 10.1007/7651_2024_574 doi pmid:39570548 pmid 1064-3745 ISSN 1940-6029 ISSN DZNE-2025-00921 English 570 Grass, Tobias P:(DE-2719)9000564 0 First author dzne Neuromuscular Organoids to Study Spinal Cord Development and Disease. New York, NY 2025 Springer US Organoids / Turksen, Kursad (Editor) ; New York, NY : Springer US, 2025, Chapter 574 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-4681-6=978-1-0716-4682-3 ; doi:10.1007/978-1-0716-4682-3 197 - 219 BOOK_CHAPTER ORCID Book Section 7 EndNote bookPart DRIVER INBOOK BibTeX Output Types/Book chapter DataCite Contribution to a book contb contb PUB:(DE-HGF)7 1756201732_31524 PUB:(DE-HGF) Methods in Molecular Biology 2951 Many aspects of neurodegenerative disease pathology remain unresolved. Why do certain neuronal subpopulations acquire vulnerability to stress or mutations in ubiquitously expressed genes, while others remain resilient? Do these neurons harbor intrinsic marks that make them prone to degeneration? Do these diseases have a neurodevelopmental component? Lacking this fundamental knowledge hampers the discovery of efficacious treatments. While it is well established that human organoids enable the modeling of brain-related diseases, we still lack an organoid model that recapitulates the regionalization complexity and physiology of the spinal cord. Here, we describe an advanced experimental protocol to generate neuromuscular organoids composed of a wide rostro-caudal (RC) diversity of spinal motor neurons (spMNs) and mesodermal progenitor-derived muscle cells. This model therefore allows for the robust and reproducible study of neuromuscular unit development and disease. 352 - Disease Mechanisms (POF4-352) G:(DE-HGF)POF4-352 POF4-352 POF IV 0 Dataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de Human induced pluripotent stem cells Other Neuromesodermal progenitors Other Neuromuscular spinal cord organoids Other Skeletal and smooth muscle Other Spinal motor neurons Other Organoids: cytology MeSH Organoids: metabolism MeSH Spinal Cord: cytology MeSH Spinal Cord: growth & development MeSH Spinal Cord: pathology MeSH Humans MeSH Motor Neurons: cytology MeSH Motor Neurons: metabolism MeSH Animals MeSH Mice MeSH Neuromuscular Junction MeSH Dokuzluoglu, Zeynep P:(DE-2719)9002364 1 dzne Rodríguez-Muela, Natalia P:(DE-2719)9000726 2 Last author dzne 10.1007/7651_2024_574 http://pub.dzne.de/record/280243/files/DZNE-2025-00921_Restricted.pdf http://pub.dzne.de/record/280243/files/DZNE-2025-00921_Restricted.pdf?subformat=pdfa pdfa oai:pub.dzne.de:280243 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)9000564 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)9002364 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)9000726 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-352 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Disease Mechanisms 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2024-12-19 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2024-12-19 I:(DE-2719)1713001 AG Rodriguez-Muela Selective Neuronal Vulnerability in Neurodegenerative Diseases 0 contb VDB I:(DE-2719)1713001 UNRESTRICTED