281358 20260309111157.0 10.1038/s44318-025-00516-y doi pmid:40813925 pmid pmc:PMC12436662 pmc 0261-4189 ISSN 1460-2075 ISSN altmetric:180316241 altmetric DZNE-2025-01105 English 570 Chhabra, Akshita P:(DE-2719)9002015 0 First author dzne Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization. [London] 2025 Nature Publishing Group UK article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1759826604_17321 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote Neuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Reorganization of SVs, synapsins, and actin is a hallmark of synaptic activity, but the molecular details of the interactions between these components remain unclear. Here, we combine in vitro reconstitution with expansion microscopy, super-resolution imaging, and cryo-electron tomography to dissect the roles of SV-synapsin-1 condensates in the organization of the presynaptic actin cytoskeleton. Our results indicate that condensation of synapsin-1 initiates actin polymerization. This process enables SV-synapsin-actin assemblies to facilitate the mesoscale organization of SV clusters along axons, which is similar to the native presynaptic organization observed at both lamprey and mammalian synapses. Understanding the relationship between the actin network and synapsin-synaptic vesicle condensates can help elucidate how coordinated neurotransmission along the axon enables circuit function and behavior. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Actin Other Liquid-Liquid Phase Separation Other Presynapses Other Synapsin Other Synaptic Vesicles Other Synapsins NLM Chemicals Actins NLM Chemicals Synaptic Vesicles: metabolism MeSH Synaptic Vesicles: ultrastructure MeSH Synapsins: metabolism MeSH Animals MeSH Actins: metabolism MeSH Polymerization MeSH Actin Cytoskeleton: metabolism MeSH Synapses: metabolism MeSH Hoffmann, Christian P:(DE-2719)9000582 1 First author Aguilar Perez, Gerard P:(DE-2719)9002012 2 First author dzne Korobeinikov, Aleksandr A P:(DE-2719)9002676 3 Rentsch, Jakob 0000-0002-5728-7255 4 Hümpfer, Nadja 0000-0002-4349-4886 5 Kokwaro, Linda P:(DE-2719)9001437 6 Gnidovec, Luka P:(DE-2719)9002532 7 dzne Petrović, Arsen 0000-0003-4448-4230 8 Wallace, Jaqulin N 0000-0001-5150-9390 9 Tromm, Johannes Vincent P:(DE-2719)9002092 10 Román-Vendrell, Cristina 0000-0003-1613-9130 11 Johnson, Emma C 0009-0005-6288-0546 12 Ranković, Branislava P:(DE-2719)9001749 13 Perego, Eleonora 14 Volpi, Tommaso 0000-0002-5451-6710 15 Fernández-Busnadiego, Rubén 0000-0002-8366-7622 16 Köster, Sarah 0000-0002-0009-1024 17 Rizzoli, Silvio O 0000-0002-1667-7839 18 Ewers, Helge 0000-0003-3948-4332 19 Morgan, Jennifer R 0000-0001-9915-6360 20 Milovanovic, Dragomir P:(DE-2719)9000670 21 Last author dzne 10.1038/s44318-025-00516-y Vol. 44, no. 18, p. 5112 - 5148 PERI:(DE-600)1467419-1 18 5112 - 5148 The EMBO journal 44 2025 0261-4189 http://pub.dzne.de/record/281358/files/DZNE-2025-01105%20SUP.zip OpenAccess http://pub.dzne.de/record/281358/files/DZNE-2025-01105.pdf OpenAccess pdfa http://pub.dzne.de/record/281358/files/DZNE-2025-01105.pdf?subformat=pdfa #741 6717.15 APC 2026-03-09 DEAL 2025-12-03 oai:pub.dzne.de:281358 openaire open_access OpenAPC driver VDB openCost dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)9002015 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)9000582 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)9002012 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)9002676 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)9001437 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 7 P:(DE-2719)9002532 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 10 P:(DE-2719)9002092 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 13 P:(DE-2719)9001749 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 21 P:(DE-2719)9000670 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2024-12-30 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2024-12-30 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2024-12-30 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2024-12-30 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2024-12-30 OpenAccess StatID:(DE-HGF)0510 StatID DEAL Wiley StatID:(DE-HGF)3001 StatID 2024-12-30 ger IF >= 10 StatID:(DE-HGF)9910 StatID EMBO J : 2022 2024-12-30 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2024-04-08T07:44:01Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2024-04-08T07:44:01Z DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2024-12-30 Fees StatID:(DE-HGF)0700 StatID 2024-12-30 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2024-12-30 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2024-12-30 Peer Review StatID:(DE-HGF)0030 StatID ASC 2024-12-30 Article Processing Charges StatID:(DE-HGF)0561 StatID 2024-12-30 JCR StatID:(DE-HGF)0100 StatID EMBO J : 2022 2024-12-30 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2024-12-30 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2024-12-30 APC keys set APC PC:(DE-HGF)0000 Local Funding APC PC:(DE-HGF)0001 DFG OA Publikationskosten APC PC:(DE-HGF)0002 DOAJ Journal APC PC:(DE-HGF)0003 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 FullTexts journal VDB UNRESTRICTED I:(DE-2719)1813002 APC