README 
Overview

This archive contains the source numerical data underlying all figures of the manuscript:

Title: The mitochondrial unfolded protein response disrupts neuronal–glial communication and promotes senescence in human microglia
Authors: Maria Jose Perez J., Alicia Lam, Christin Weissleder, Federico Bertoli, Hariam Raji, Mariella Bosch, Ivan Nemazanyy, Stefanie Kalb, Mohammed Kehili, Insa Hirschberg, Dario Brunetti, Indra Heckenbach, Morten Scheibye-Knudsen, and Michela Deleidi
Date: October 2025
Corresponding authors: Pérez M.J., Deleidi M., et al. (Imagine Institute, Paris)

Each file corresponds to the exact dataset plotted in the figures.

File Naming Convention

Files are named as:

Main_M[X][PanelLetter]_Zenodo.xlsx
Supplementary_S[X][PanelLetter]_Zenodo.xlsx


Main / Supplementary → Indicates whether data belong to main or supplementary figures.

M[X] / S[X] → Figure number (e.g., M2 = Main Figure 2).

PanelLetter → Specific figure panel (e.g., M2D = panel D of Figure 2).

_Zenodo → Indicates that this version corresponds to the deposited open-access data.

File Structure and Content

Each Excel file typically contains:

Raw or normalized data values used to generate the plots shown in the corresponding figure panel.

Column headers describing experimental conditions, sample IDs, or replicates.

Units and normalization methods specified in the figure legend or Methods section of the paper.

Data Types

Depending on the figure, the tables may include:

qPCR, Western blot quantification, immunofluorescence signal intensity

RNA-seq / scRNA-seq summary data (processed counts, normalized expression, or pathway enrichment values)

Metabolomics and lipidomics processed quantifications

Flow cytometry quantifications (mean fluorescence intensity, % positive cells, etc.)

Image-derived quantifications (area, mean intensity, number of positive cells per field, etc.)

Usage

All files are provided as .xlsx to ensure accessibility and direct compatibility with most spreadsheet or analysis software.
Users are encouraged to refer to the Methods and Figure Legends of the manuscript for full experimental context and data interpretation.

Notes

Each file corresponds exactly to the dataset plotted in the figure panel.

No additional normalization or transformation has been applied beyond what is described in the manuscript.
