TY - JOUR
AU - Vasili, Eftychia
AU - König, Annekatrin
AU - Al-Azzani, Mohammed
AU - Bosbach, Clara
AU - Gatzemeier, Luisa Maria
AU - Thom, Searlait
AU - Chegão, Ana
AU - Miranda, Hugo Vicente
AU - Steinem, Claudia
AU - Erskine, Daniel
AU - Outeiro, Tiago F
TI - Glycation of alpha-synuclein enhances aggregation and neuroinflammatory responses.
JO - npj Parkinson's Disease
VL - 11
IS - 1
SN - 2373-8057
CY - [London]
PB - Springer Nature
M1 - DZNE-2025-01195
SP - 307
PY - 2025
AB - The risk of developing Parkinson's disease (PD) is elevated in individuals with type 2 diabetes (T2DM), but the molecular pathways underlying this link remain unclear. Glycation, a non-enzymatic modification of lysine and arginine residues by reducing sugars or reactive dicarbonyls, may disrupt proteostasis and trigger pathology. Here, we investigated how methylglyoxal (MGO)- and ribose-mediated glycation influence aSyn aggregation, neuroinflammation, and detoxification pathways. Using SH-SY5Y cells, primary neurons, primary microglia and MGO-injected aSyn transgenic mice, we found that MGO-glycated aSyn promotes PD associated pathological features, including pS129-positive aSyn aggregates, neuroinflammation, and impairment of the glyoxalase detoxification pathway. Ribose-glycated aSyn, while immunogenic, exerts limited effects on aggregation and seeding. Both glycated species activates microglia and upregulate pro-inflammatory markers. We further developed a novel antibody specific for MGO-glycated aSyn, which selectively detects Lewy body-like deposits in dementia with Lewy bodies (DLB) tissue and MGO-injected mice. These findings implicate MGO-glycation in PD-T2DM comorbidity.
LB - PUB:(DE-HGF)16
C6 - pmid:41131060
DO - DOI:10.1038/s41531-025-01159-w
UR - https://pub.dzne.de/record/281812
ER -