282294 20251212103343.0 10.1186/s12915-025-02464-9 doi pmid:41239319 pmid pmc:PMC12619239 pmc DZNE-2025-01264 English 610 Mimoso, Tiago 0 Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin. Heidelberg 2025 Springer article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1765455324_17917 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years, evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonetheless, one question remains open, namely whether the vesicles influence the availability of tubulin within the synaptic compartment.An analysis of previously published live imaging experiments indicates that tubulin is strongly enriched in the synaptic vesicle cluster. To analyze the vesicle-tubulin interaction directly, we isolated vesicles from the mouse brain and imaged them together with fluorescent tubulin in vitro. We found that soluble tubulin is collected by synaptic vesicles in physiological buffers, resulting in the formation of tubulin-rich regions (TRRs) on the respective vesicle clusters.We conclude that the synaptic vesicle cluster is indeed able to recruit soluble tubulin. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Cytoskeleton Other Microtubules Other Synapse Other Synaptic vesicle cluster Other Synaptic vesicles Other Tubulin Other Tubulin NLM Chemicals Animals MeSH Tubulin: metabolism MeSH Synaptic Vesicles: metabolism MeSH Mice MeSH Brain: metabolism MeSH Microtubules: metabolism MeSH Korobeinikov, Aleksandr P:(DE-2719)9002676 1 dzne Stein, Alexander 2 Milovanovic, Dragomir P:(DE-2719)9000670 3 dzne Rizzoli, Silvio O 4 Köster, Sarah 5 Reshetniak, Sofiia 6 10.1186/s12915-025-02464-9 Vol. 23, no. 1, p. 340 PERI:(DE-600)2133020-7 1 340 BMC biology 23 2025 1741-7007 http://pub.dzne.de/record/282294/files/DZNE-2025-01264%20SUP.pdf OpenAccess http://pub.dzne.de/record/282294/files/DZNE-2025-01264.pdf pdfa http://pub.dzne.de/record/282294/files/DZNE-2025-01264%20SUP.pdf?subformat=pdfa OpenAccess pdfa http://pub.dzne.de/record/282294/files/DZNE-2025-01264.pdf?subformat=pdfa oai:pub.dzne.de:282294 openaire open_access VDB driver dnbdelivery Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)9002676 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)9000670 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2025 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2024-12-09 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2024-12-09 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2024-12-09 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2024-12-09 DBCoverage StatID:(DE-HGF)0600 StatID Ebsco Academic Search 2024-12-09 DBCoverage StatID:(DE-HGF)1040 StatID Zoological Record 2024-12-09 JCR StatID:(DE-HGF)0100 StatID BMC BIOL : 2022 2024-12-09 DBCoverage StatID:(DE-HGF)0501 StatID DOAJ Seal 2024-04-10T15:34:04Z DBCoverage StatID:(DE-HGF)0500 StatID DOAJ 2024-04-10T15:34:04Z WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2024-12-09 Fees StatID:(DE-HGF)0700 StatID 2024-12-09 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2024-12-09 OpenAccess StatID:(DE-HGF)0510 StatID Peer Review StatID:(DE-HGF)0030 StatID ASC 2024-12-09 Article Processing Charges StatID:(DE-HGF)0561 StatID 2024-12-09 IF >= 5 StatID:(DE-HGF)9905 StatID BMC BIOL : 2022 2024-12-09 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2024-12-09 Creative Commons Attribution CC BY 4.0 LIC:(DE-HGF)CCBY4 HGFVOC DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2024-12-09 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 I:(DE-2719)1013043 AG Milovanovic (Bonn) Molecular Neuroscience 1 journal VDB UNRESTRICTED I:(DE-2719)1813002 I:(DE-2719)1013043 FullTexts