282939 20251218103447.0 10.17632/W97NRDVXBC.1 doi 10.17632/w97nrdvxbc.1 doi DZNE-2025-01400 Kroon, Cristina 0 Dataset: Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release 2025 Mendeley Data MISC BibTeX Dataset dataset dataset PUB:(DE-HGF)32 1765985150_5348 PUB:(DE-HGF) Chart or Table 26 EndNote Dataset DataCite DATA_SET ORCID ResearchData DINI This dataset contains the raw data from the publication „Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release“ Kroon et al. 2025 published in iScience. Presented here are the original western and PhosTag blots supporting the biochemical characterization, as well as mass spectrometry and immunocytochemistry data. For further details please refer to the publication. Abstract: Phospholipid-phosphatase related protein 3 (PLPPR3) belongs to a family of transmembrane proteins highly expressed in the nervous system where it regulates critical axonal growth processes during guidance, filopodia formation and branching. However, little is known regarding its role in synapses and the signaling events regulating PLPPR3 function. Here, we identify 26 high-confidence phosphorylation sites in the intracellular domain of PLPPR3 using mass spectrometry. Biochemical characterization established one of these – S351 – as a bona fide phosphorylation site of protein kinase A (PKA). PLPPR3 is enriched at presynaptic terminals, and deletion of PLPPR3 results in increased depolarization-induced synaptic vesicle release in hippocampal neurons. This tonic inhibitory signal towards depolarization-induced presynaptic activity is corrected by expression of PLPPR3 intracellular domain, but not a S351A phospho-dead mutant, in Plppr3-/- hippocampal neurons. We propose that a PLPPR3 phosphorylation under the control of PKA activity is a novel signaling integrator of presynaptic activity in hippocampal neurons. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to DataCite Molecular Neuroscience Other Phosphorylation Other Protein-Protein Interaction Other Signal Transduction Other Cellular Neuroscience Other Presynaptic Mechanisms of Synaptic Transmission Other Protein Phosphorylation Other Nagy-Herczeg, Domonkos 1 Aguilar Perez, Gerard P:(DE-2719)9002012 2 dzne Ranti, Dimitra 3 Syropoulou, Vasiliki 4 10.17632/w97nrdvxbc.1 Kroon, Cristina et.al. St. Louis : Elsevier, 2025 RelatedTo DZNE-2025-01117 Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release oai:pub.dzne.de:282939 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)9002012 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2025 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 dataset VDB I:(DE-2719)1813002 UNRESTRICTED