283031 20260107144732.0 DZNE-2025-01443 Chhabra, Akshita P:(DE-2719)9002015 0 dzne Condensates of synaptic vesicles and synapsin-1 mediate actin polymerization 2025 BioStudies MISC BibTeX Dataset dataset dataset PUB:(DE-HGF)32 1767355570_32511 PUB:(DE-HGF) Chart or Table 26 EndNote Dataset DataCite DATA_SET ORCID ResearchData DINI Deutsche Forschungsgemeinschaft (DFG): MI 2104 SFB 1286/B10 SFB 1286/B02 SFB 1286/B02EC | ERC | HORIZON EUROPE European Research Council (ERC): 101078172HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS): NS078165-12 NS126575-01Deutsche Demenzhilfe: Innovative Minds ProgramGerman Academic Scholarship Foundation: N/A Neuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Reorganization of SVs, synapsins, and actin is a hallmark of synaptic activity, but the molecular details of the interactions between these components remain unclear. Here, we combine in vitro reconstitution with expansion microscopy, super-resolution imaging, and cryo-electron tomography to dissect the roles of SV-synapsin-1 condensates in the organization of the presynaptic actin cytoskeleton. Our results indicate that condensation of synapsin-1 initiates actin polymerization. This process enables SV-synapsin-actin assemblies to facilitate the mesoscale organization of SV clusters along axons, which is similar to the native presynaptic organization observed at both lamprey and mammalian synapses. Understanding the relationship between the actin network and synapsin-synaptic vesicle condensates can help elucidate how coordinated neurotransmission along the axon enables circuit function and behavior. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Hoffmann, Christian P:(DE-2719)9000582 1 dzne Aguilar Perez, Gerard P:(DE-2719)9002012 2 dzne Korobeinikov, Aleksandr P:(DE-2719)9002676 3 dzne Rentsch, Jakob P:(DE-HGF)0 4 Hümpfer, Nadja P:(DE-HGF)0 5 Kokwaro, Linda P:(DE-2719)9001437 6 Gnidovec, Luka P:(DE-2719)9002532 7 dzne Petrovic, Arsen P:(DE-HGF)0 8 Wallace, Jaqulin P:(DE-HGF)0 9 Tromm, Johannes Vincent P:(DE-2719)9002092 10 dzne Roman-Vendrell, Cristina P:(DE-HGF)0 11 Johnson, Emma P:(DE-HGF)0 12 Rankovic, Branislava P:(DE-2719)9001749 13 dzne Perego, Eleonora P:(DE-HGF)0 14 Volpi, Tommaso P:(DE-HGF)0 15 Fernandez-Busnadiego, Ruben P:(DE-HGF)0 16 Köster, Sarah P:(DE-HGF)0 17 Rizzoli, Silvio P:(DE-HGF)0 18 Ewers, Helge P:(DE-HGF)0 19 Morgan, Jennifer P:(DE-HGF)0 20 Milovanovic, Dragomir P:(DE-2719)9000670 21 Last author dzne Chhabra, Akshita et.al. [London] : Nature Publishing Group UK, 2025 RelatedTo DZNE-2025-01105 Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization. https://www.ebi.ac.uk/biostudies/sourcedata/studies/S-SCDT-10_1038-S44318-025-00516-y oai:pub.dzne.de:283031 VDB Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 0 P:(DE-2719)9002015 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 1 P:(DE-2719)9000582 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 2 P:(DE-2719)9002012 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 3 P:(DE-2719)9002676 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 6 P:(DE-2719)9001437 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 7 P:(DE-2719)9002532 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 10 P:(DE-2719)9002092 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 13 P:(DE-2719)9001749 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 21 P:(DE-2719)9000670 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 2025 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 I:(DE-2719)1013043 AG Milovanovic (Bonn) Molecular Neuroscience 1 dataset VDB I:(DE-2719)1813002 I:(DE-2719)1013043 UNRESTRICTED