47 Emery, Brett Addison Khanzada, Shahrukh Hu, Xin Amin, Hayder AG Amin 2025 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) : [Proceedings] - IEEE, 2025. - ISBN 979-8-3315-8618-8 - doi:10.1109/EMBC58623.2025.11253841 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society IEEE Copenhagen, Denmark 10.1109/EMBC58623.2025.11253841 English 1-4 70 million people suffer from epilepsy worldwide with temporal lobe epilepsy (TLE) being the most common focal epilepsy in adults. The hippocampus, a region associated with TLE, is highly susceptible to pathological synchronization and epileptogenesis due to its functional and anatomical organization. Despite progress, understanding the distinct contributions of hippocampal subregions to multiplexed activation and hyperactivity remains challenging due to the interplay of receptor dynamics, ion channel regulation, and network synchronization. Here, a large-scale ex-vivo electrophysiological platform was implemented using a high-density CMOS-based biosensor (HD-MEA) to probe the spatiotemporal dynamics of 4AP and ATPA, benchmark compounds for inducing hyperactivity, within the hippocampal circuit. This approach demonstrates the use of large-scale biosensors to address these challenges by enabling high-resolution spatiotemporal mapping of circuit activity, demonstrating their potential to uncover ion channel and receptor-specific spatiotemporal signatures of hyperactivity. Missing Journal: Annu Int Conf IEEE Eng Med Biol Soc = 2375-7477 (import from CrossRef Conference, PubMed, , Journals: pub.dzne.de) ; 2025 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) : [Proceedings] - IEEE, 2025. - ISBN 979-8-3315-8618-8 - doi:10.1109/EMBC58623.2025.11253841 ; 33, ; PUB:(DE-HGF)8, ; PUB:(DE-HGF)7, ; Biosensing Techniques: methods Biosensing Techniques: instrumentation Hippocampus: physiopathology Animals Epilepsy: physiopathology Epilepsy, Temporal Lobe: physiopathology Spatio-Temporal Analysis Humans Contribution to a conference proceedings 2025 2025-07-14 - 2025-07-18 DZNE-2026-00342 2025-07-14 - 2025-07-18 2025 pmid:41337179 https://pub.dzne.de/record/285806 https://doi.org/10.1109/EMBC58623.2025.11253841