47 Emery, Brett Addison Khanzada, Shahrukh Hu, Xin Maggi, Maria Anna Bisti, Silvia Amin, Hayder AG Amin 2025 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) : [Proceedings] - IEEE, 2025. - ISBN 979-8-3315-8618-8 - doi:10.1109/EMBC58623.2025.11253272 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society IEEE Copenhagen, Denmark 10.1109/EMBC58623.2025.11253272 English 1-4 Alzheimer's disease (AD), a progressive neurodegenerative disorder, is projected to affect over 130 million people globally by 2050. While extensive efforts have focused on targeting molecular hallmarks such as amyloid-beta (Aβ) plaques and tau pathology, network-level dysfunction remains a critical but underexplored component of AD progression. Disruptions in hippocampal-cortical (HC) circuit activity emerge early in AD, compromising memory processing and cognitive functions. Characterizing these disruptions requires high-resolution platforms capable of capturing network-wide spatiotemporal dynamics. To address this, we implemented a high-density microelectrode array (HD-MEA) biosensor to assess large-scale electrophysiological activity in ex vivo hippocampal slices from well-established APPNL and APPNL-G-F mouse models. Our approach quantifies hippocampal oscillatory disturbances and examines their modulation by saffron, a natural compound with reported neuroprotective properties. Results indicate that hippocampal network activity is progressively impaired in APPNL-G-F mice, particularly in sharp-wave ripple (SWR) and multi-unit activity (MUA) patterns. The HD-MEA platform provides a scalable tool for investigating AD-associated network dysfunctions and exploring potential modulatory interventions. Missing Journal: Annu Int Conf IEEE Eng Med Biol Soc = 2375-7477 (import from CrossRef Conference, PubMed, , Journals: pub.dzne.de) ; 2025 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) : [Proceedings] - IEEE, 2025. - ISBN 979-8-3315-8618-8 - doi:10.1109/EMBC58623.2025.11253272 ; 33, ; PUB:(DE-HGF)8, ; PUB:(DE-HGF)7, ; Alzheimer Disease: physiopathology Hippocampus: physiopathology Hippocampus: pathology Animals Mice Mice, Transgenic Disease Models, Animal Microelectrodes Electrophysiology: methods Nerve Net: physiopathology Humans Contribution to a conference proceedings 2025 2025-07-14 - 2025-07-18 DZNE-2026-00345 2025-07-14 - 2025-07-18 2025 pmid:41335966 https://pub.dzne.de/record/285809 https://doi.org/10.1109/EMBC58623.2025.11253272