286088 20260413114003.0 10.1016/j.bpj.2026.03.006 doi pmid:41795188 pmid 0006-3495 ISSN 1542-0086 ISSN DZNE-2026-00384 English 570 Czajka, Titus 0 Vesicle dynamics in synapsin-induced condensates by passive X-ray microrheology. Cambridge, Mass. 2026 Cell Press article DRIVER Output Types/Journal article DataCite Journal Article journal journal PUB:(DE-HGF)16 1776073120_13395 PUB:(DE-HGF) ARTICLE BibTeX JOURNAL_ARTICLE ORCID Journal Article 0 EndNote The collective dynamics of subcellular biological processes is often difficult to assess experimentally due to the challenges associated with spatial and temporal resolution, labeling, or multiple scattering. X-ray photon correlation spectroscopy is, in principle, well suited to probe collective dynamics by quantifying dispersion relations in complex fluids in general and biomolecular systems in particular. However, the low scattering signal and the sensitivity to radiation damage set stringent limits to many applications. Probing the dynamics of vesicles in protein-induced condensates is a case in point. Here, we use lipid vesicles with a hard silica core, called colloid-supported lipid bilayers, as labeled vesicles for enhanced X-ray contrast. We then probe structure and dynamics in solutions of vesicles and synapsin, a protein known for its property of inducing liquid-liquid phase separation and forming condensates that recruit vesicles, organizing them into clusters in presynaptic nerve terminals. The dynamics in these systems is found to exhibit evidence for both liquid-like and network-like phases. Our results reveal distinct effective-diffusion constants at varying protein concentrations. At the same time the stretched exponential decay of the correlation functions provides clear evidence for nondiffusive behavior within the condensates. 351 - Brain Function (POF4-351) G:(DE-HGF)POF4-351 POF4-351 POF IV 0 Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de Lipid Bilayers NLM Chemicals Synapsins NLM Chemicals Lipid Bilayers: chemistry MeSH Lipid Bilayers: metabolism MeSH Synapsins: metabolism MeSH Synapsins: chemistry MeSH Diffusion MeSH X-Rays MeSH Biomolecular Condensates: chemistry MeSH Biomolecular Condensates: metabolism MeSH Major, Andras 1 Bruns, Hendrik 2 Cammarata, Marco 3 Hoffmann, Christian P:(DE-2719)9000582 4 dzne Milovanovic, Dragomir P:(DE-2719)9000670 5 dzne Salditt, Tim 6 10.1016/j.bpj.2026.03.006 Vol. 125, no. 7, p. 1713 - 1722 PERI:(DE-600)1477214-0 7 1713 - 1722 Biophysical journal 125 2026 0006-3495 http://pub.dzne.de/record/286088/files/DZNE-2026-00384.pdf Restricted http://pub.dzne.de/record/286088/files/DZNE-2026-00384.pdf?subformat=pdfa pdfa Restricted Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 4 P:(DE-2719)9000582 Deutsches Zentrum für Neurodegenerative Erkrankungen I:(DE-588)1065079516 DZNE 5 P:(DE-2719)9000670 DE-HGF Gesundheit Neurodegenerative Diseases G:(DE-HGF)POF4-350 G:(DE-HGF)POF4-351 G:(DE-HGF)POF4 G:(DE-HGF)POF4-300 G:(DE-HGF)POF Brain Function 0 JCR StatID:(DE-HGF)0100 StatID BIOPHYS J : 2022 2025-11-12 DBCoverage StatID:(DE-HGF)0200 StatID SCOPUS 2025-11-12 DBCoverage StatID:(DE-HGF)0300 StatID Medline 2025-11-12 DBCoverage StatID:(DE-HGF)0320 StatID PubMed Central 2025-11-12 DBCoverage StatID:(DE-HGF)0199 StatID Clarivate Analytics Master Journal List 2025-11-12 DBCoverage StatID:(DE-HGF)1190 StatID Biological Abstracts 2025-11-12 DBCoverage StatID:(DE-HGF)0160 StatID Essential Science Indicators 2025-11-12 WoS StatID:(DE-HGF)0113 StatID Science Citation Index Expanded 2025-11-12 DBCoverage StatID:(DE-HGF)0150 StatID Web of Science Core Collection 2025-11-12 DBCoverage StatID:(DE-HGF)1030 StatID Current Contents - Life Sciences 2025-11-12 DBCoverage StatID:(DE-HGF)1050 StatID BIOSIS Previews 2025-11-12 IF < 5 StatID:(DE-HGF)9900 StatID 2025-11-12 I:(DE-2719)1813002 AG Milovanovic (Berlin) Molecular Neuroscience 0 journal EDITORS VDBINPRINT I:(DE-2719)1813002 UNRESTRICTED