% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Lopes:284084,
      author       = {Lopes, Alexandre H. and Silva, Rangel L. and Fonseca,
                      Miriam D. and Gomes, Francisco I. and Maganin, Alexandre G.
                      and Secchim Ribeiro, Lucas and Marques, Lucas Maciel Mauriz
                      and Cunha, Fernando Q. and Alves-Filho, Jose C. and Zamboni,
                      Dario S. and Lopes, Norberto P. and Franklin, Bernardo S.
                      and Gombault, Aurélie and Ramalho, Fernando Silva and
                      Quesniaux, Valerie F. J. and Couillin, Isabelle and Ryffel,
                      Bernhard and Cunha, Thiago M.},
      title        = {{M}olecular basis of carrageenan-induced cytokines
                      production in macrophages},
      journal      = {Cell communication and signaling},
      volume       = {18},
      number       = {1},
      issn         = {1478-811X},
      address      = {London},
      publisher    = {Biomed Central},
      reportid     = {DZNE-2026-00092},
      pages        = {141},
      year         = {2020},
      abstract     = {Low molecular weight carrageenan (Cg) is a seaweed-derived
                      sulfated polysaccharide widely used as inflammatory stimulus
                      in preclinical studies. However, the molecular mechanisms of
                      Cg-induced inflammation are not fully elucidated. The
                      present study aimed to investigate the molecular basis
                      involved in Cg-induced macrophages activation and cytokines
                      production.Primary culture of mouse peritoneal macrophages
                      were stimulated with Kappa Cg. The supernatant and cell
                      lysate were used for ELISA, western blotting,
                      immunofluorescence. Cg-induced mouse colitis was also
                      developed.Here we show that Cg activates peritoneal
                      macrophages to produce pro-inflammatory cytokines such as
                      TNF and IL-1β. While Cg-induced TNF production/secretion
                      depends on TLR4/MyD88 signaling, the production of
                      pro-IL-1β relies on TLR4/TRIF/SYK/reactive oxygen species
                      (ROS) signaling pathway. The maturation of pro-IL1β into
                      IL-1β is dependent on canonical NLRP3 inflammasome
                      activation via Pannexin-1/P2X7/K+ efflux signaling. In vivo,
                      Cg-induced colitis was reduced in mice in the absence of
                      NLRP3 inflammasome components.In conclusion, we unravel a
                      critical role of the NLRP3 inflammasome in Cg-induced
                      pro-inflammatory cytokines production and colitis, which is
                      an important discovery on the pro-inflammatory properties of
                      this sulfated polysaccharide for pre-clinical studies. Video
                      abstract Carrageenan (Cg) is one the most used flogistic
                      stimulus in preclinical studies. Nevertheless, the molecular
                      basis of Cg-induced inflammation is not totally elucidated.
                      Herein, Lopes et al. unraveled the molecular basis for
                      Cg-induced macrophages production of biological active
                      IL-1β. The Cg-stimulated macrophages produces pro-IL-1β
                      depends on TLR4/TRIF/Syk/ROS, whereas its processing into
                      mature IL-1β is dependent on the canonical NLRP3
                      inflammasome.},
      keywords     = {Animals / Carrageenan: immunology / Cells, Cultured /
                      Cytokines: immunology / Inflammasomes: immunology /
                      Inflammation: immunology / Interleukin-1beta: immunology /
                      Macrophage Activation / Macrophages, Peritoneal: immunology
                      / Male / Mice / Mice, Inbred C57BL / NLR Family, Pyrin
                      Domain-Containing 3 Protein: immunology / Tumor Necrosis
                      Factor-alpha: immunology / Carrageenan (Other) / IL-1β
                      (Other) / Macrophages (Other) / NLRP3 Inflammasome (Other) /
                      Pannexin-1 channel (Other) / Cytokines (NLM Chemicals) /
                      IL1B protein, mouse (NLM Chemicals) / Inflammasomes (NLM
                      Chemicals) / Interleukin-1beta (NLM Chemicals) / NLR Family,
                      Pyrin Domain-Containing 3 Protein (NLM Chemicals) / Nlrp3
                      protein, mouse (NLM Chemicals) / Tumor Necrosis Factor-alpha
                      (NLM Chemicals) / Carrageenan (NLM Chemicals)},
      ddc          = {570},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1186/s12964-020-00621-x},
      url          = {https://pub.dzne.de/record/284084},
}