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@ARTICLE{Oliveira:284089,
author = {Oliveira, Lorena V. N. and Costa, Marliete C. and
Magalhães, Thaís F. F. and Bastos, Rafael W. and Campi
Santos, Patricia and Carneiro, Hellem C. S. and Ribeiro,
Noelly Q. and Ferreira, Gabriella F. and Secchim Ribeiro,
Lucas and Gonçalves, Ana P. F. and Fagundes, Caio T. and
Pascoal-Xavier, Marcelo A. and Djordjevic, Julianne T. and
Sorrell, Tania C. and Souza, Daniele G. and Machado,
Alexandre M. V. and Santos, Daniel A.},
title = {{I}nfluenza {A} {V}irus as a {P}redisposing {F}actor for
{C}ryptococcosis},
journal = {Frontiers in Cellular and Infection Microbiology},
volume = {7},
issn = {2235-2988},
address = {Lausanne},
publisher = {Frontiers Media},
reportid = {DZNE-2026-00097},
pages = {419},
year = {2017},
abstract = {Influenza A virus (IAV) infects millions of people annually
and predisposes to secondary bacterial infections.
Inhalation of fungi within the Cryptococcus complex causes
pulmonary disease with secondary meningo-encephalitis.
Underlying pulmonary disease is a strong risk factor for
development of C. gattii cryptococcosis though the effect of
concurrent infection with IAV has not been studied. We
developed an in vivo model of Influenza A H1N1 and C. gattii
co-infection. Co-infection resulted in a major increase in
morbidity and mortality, with severe lung damage and a high
brain fungal burden when mice were infected in the acute
phase of influenza multiplication. Furthermore, IAV alters
the host response to C. gattii, leading to recruitment of
significantly more neutrophils and macrophages into the
lungs. Moreover, IAV induced the production of type 1
interferons (IFN-α4/β) and the levels of IFN-γ were
significantly reduced, which can be associated with
impairment of the immune response to Cryptococcus during
co-infection. Phagocytosis, killing of cryptococci and
production of reactive oxygen species (ROS) by IAV-infected
macrophages were reduced, independent of previous IFN-γ
stimulation, leading to increased proliferation of the
fungus within macrophages. In conclusion, IAV infection is a
predisposing factor for severe disease and adverse outcomes
in mice co-infected with C. gattii.},
keywords = {Acetylglucosaminidase: metabolism / Animals / Behavior,
Animal / Brain: microbiology / Brain: pathology / Causality
/ Cell Proliferation / Chemokines: metabolism / Coinfection:
immunology / Coinfection: microbiology / Coinfection:
mortality / Coinfection: virology / Cryptococcosis:
complications / Cryptococcosis: immunology / Cryptococcus
gattii: immunology / Cryptococcus gattii: pathogenicity /
Cryptococcus neoformans: immunology / Cytokines: metabolism
/ Disease Models, Animal / Disease Susceptibility / Dogs /
Female / Humans / Influenza A Virus, H1N1 Subtype:
immunology / Influenza A Virus, H1N1 Subtype: pathogenicity
/ Interferon-gamma: metabolism / Lung: enzymology / Lung:
pathology / Lung: virology / Macrophages: metabolism /
Macrophages: virology / Madin Darby Canine Kidney Cells /
Mice / Mice, Inbred C57BL / Neutrophils / Nitric Oxide:
metabolism / Orthomyxoviridae Infections: complications /
Orthomyxoviridae Infections: immunology / Peroxidase:
metabolism / Peroxynitrous Acid: metabolism / Phagocytosis /
Reactive Oxygen Species: metabolism / Survival Rate /
Cryptococcosis (Other) / Cryptococcus gattii (Other) /
co-infection (Other) / influenza A H1N1 (Other) / risk
factor (Other) / Chemokines (NLM Chemicals) / Cytokines (NLM
Chemicals) / IFNG protein, mouse (NLM Chemicals) / Reactive
Oxygen Species (NLM Chemicals) / Peroxynitrous Acid (NLM
Chemicals) / Nitric Oxide (NLM Chemicals) / Interferon-gamma
(NLM Chemicals) / Peroxidase (NLM Chemicals) /
Acetylglucosaminidase (NLM Chemicals)},
ddc = {610},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
doi = {10.3389/fcimb.2017.00419},
url = {https://pub.dzne.de/record/284089},
}