TY  - JOUR
AU  - Segatto, Marcela
AU  - Secchim Ribeiro, Lucas
AU  - Costa, Dorcas Lamounier
AU  - Costa, Carlos Henrique Nery
AU  - Oliveira, Márcia Rosa de
AU  - Carvalho, Sílvio Fernando Guimarães
AU  - Macedo, Andréa Mara
AU  - Valadares, Helder Magno Silva
AU  - Dietze, Reynaldo
AU  - Brito, Cristiana Ferreira Alves de
AU  - Lemos, Elenice Moreira
TI  - Genetic diversity of Leishmania infantum field populations from Brazil
JO  - Memórias do Instituto Oswaldo Cruz
VL  - 107
IS  - 1
SN  - 0074-0276
CY  - Rio de Janeiro
PB  - Inst.
M1  - DZNE-2026-00107
SP  - 39 - 47
PY  - 2012
AB  - Leishmania infantum (syn. Leishmania chagasi) is the etiological agent of visceral leishmaniasis (VL) in Brazil. The epidemiology of VL is poorly understood. Therefore, a more detailed molecular characterization at an intraspecific level is certainly needed. Herein, three independent molecular methods, multilocus microsatellite typing (MLMT), random amplification of polymorphic DNA (RAPD) and simple sequence repeats-polymerase chain reaction (SSR-PCR), were used to evaluate the genetic diversity of 53 L. infantum isolates from five different endemic areas in Brazil. Population structures were inferred by distance-based and Bayesian-based approaches. Eighteen very similar genotypes were detected by MLMT, most of them differed in only one locus and no correlation was found between MLMT profiles, geographical origin or the estimated population structure. However, complex profiles composed of 182 bands obtained by both RAPD and SSR-PCR assays gave different results. Unweighted pair group method with arithmetic mean trees built from these data revealed a high degree of homogeneity within isolates of L. infantum. Interestingly, despite this genetic homogeneity, most of the isolates clustered according to their geographical origin.
KW  - Animals
KW  - Brazil
KW  - Cluster Analysis
KW  - DNA, Protozoan: genetics
KW  - Dogs
KW  - Genetic Variation: genetics
KW  - Genotype
KW  - Humans
KW  - Leishmania infantum: genetics
KW  - Leishmania infantum: isolation & purification
KW  - Microsatellite Repeats
KW  - Molecular Typing
KW  - Polymerase Chain Reaction
KW  - Random Amplified Polymorphic DNA Technique
KW  - DNA, Protozoan (NLM Chemicals)
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1590/S0074-02762012000100006
UR  - https://pub.dzne.de/record/284099
ER  -