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@ARTICLE{Segatto:284099,
      author       = {Segatto, Marcela and Secchim Ribeiro, Lucas and Costa,
                      Dorcas Lamounier and Costa, Carlos Henrique Nery and
                      Oliveira, Márcia Rosa de and Carvalho, Sílvio Fernando
                      Guimarães and Macedo, Andréa Mara and Valadares, Helder
                      Magno Silva and Dietze, Reynaldo and Brito, Cristiana
                      Ferreira Alves de and Lemos, Elenice Moreira},
      title        = {{G}enetic diversity of {L}eishmania infantum field
                      populations from {B}razil},
      journal      = {Memórias do Instituto Oswaldo Cruz},
      volume       = {107},
      number       = {1},
      issn         = {0074-0276},
      address      = {Rio de Janeiro},
      publisher    = {Inst.},
      reportid     = {DZNE-2026-00107},
      pages        = {39 - 47},
      year         = {2012},
      abstract     = {Leishmania infantum (syn. Leishmania chagasi) is the
                      etiological agent of visceral leishmaniasis (VL) in Brazil.
                      The epidemiology of VL is poorly understood. Therefore, a
                      more detailed molecular characterization at an intraspecific
                      level is certainly needed. Herein, three independent
                      molecular methods, multilocus microsatellite typing (MLMT),
                      random amplification of polymorphic DNA (RAPD) and simple
                      sequence repeats-polymerase chain reaction (SSR-PCR), were
                      used to evaluate the genetic diversity of 53 L. infantum
                      isolates from five different endemic areas in Brazil.
                      Population structures were inferred by distance-based and
                      Bayesian-based approaches. Eighteen very similar genotypes
                      were detected by MLMT, most of them differed in only one
                      locus and no correlation was found between MLMT profiles,
                      geographical origin or the estimated population structure.
                      However, complex profiles composed of 182 bands obtained by
                      both RAPD and SSR-PCR assays gave different results.
                      Unweighted pair group method with arithmetic mean trees
                      built from these data revealed a high degree of homogeneity
                      within isolates of L. infantum. Interestingly, despite this
                      genetic homogeneity, most of the isolates clustered
                      according to their geographical origin.},
      keywords     = {Animals / Brazil / Cluster Analysis / DNA, Protozoan:
                      genetics / Dogs / Genetic Variation: genetics / Genotype /
                      Humans / Leishmania infantum: genetics / Leishmania
                      infantum: isolation $\&$ purification / Microsatellite
                      Repeats / Molecular Typing / Polymerase Chain Reaction /
                      Random Amplified Polymorphic DNA Technique / DNA, Protozoan
                      (NLM Chemicals)},
      ddc          = {610},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1590/S0074-02762012000100006},
      url          = {https://pub.dzne.de/record/284099},
}