%0 Journal Article
%A Babu, Harish
%A Claasen, Jan-Hendrik
%A Kannan, Suresh
%A Rünker, Annette E
%A Palmer, Theo
%A Kempermann, Gerd
%T A protocol for isolation and enriched monolayer cultivation of neural precursor cells from mouse dentate gyrus.
%J Frontiers in neuroscience
%V 5
%@ 1662-4548
%C Lausanne
%I Frontiers Research Foundation
%M DZNE-2020-02307
%P 89
%D 2011
%X In vitro assays are valuable tools to study the characteristics of adult neural precursor cells under controlled conditions with a defined set of parameters. We here present a detailed protocol based on our previous original publication (Babu et al., 2007) to isolate neural precursor cells from the hippocampus of adult mice and maintain and propagate them as adherent monolayer cultures. The strategy is based on the use of Percoll density gradient centrifugation to enrich precursor cells from the micro-dissected dentate gyrus. Based on the expression of Nestin and Sox2, a culture-purity of more than 98
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:21811434
%2 pmc:PMC3140691
%R 10.3389/fnins.2011.00089
%U https://pub.dzne.de/record/135985