TY  - JOUR
AU  - Babu, Harish
AU  - Claasen, Jan-Hendrik
AU  - Kannan, Suresh
AU  - Rünker, Annette E
AU  - Palmer, Theo
AU  - Kempermann, Gerd
TI  - A protocol for isolation and enriched monolayer cultivation of neural precursor cells from mouse dentate gyrus.
JO  - Frontiers in neuroscience
VL  - 5
SN  - 1662-4548
CY  - Lausanne
PB  - Frontiers Research Foundation
M1  - DZNE-2020-02307
SP  - 89
PY  - 2011
AB  - In vitro assays are valuable tools to study the characteristics of adult neural precursor cells under controlled conditions with a defined set of parameters. We here present a detailed protocol based on our previous original publication (Babu et al., 2007) to isolate neural precursor cells from the hippocampus of adult mice and maintain and propagate them as adherent monolayer cultures. The strategy is based on the use of Percoll density gradient centrifugation to enrich precursor cells from the micro-dissected dentate gyrus. Based on the expression of Nestin and Sox2, a culture-purity of more than 98
LB  - PUB:(DE-HGF)16
C6  - pmid:21811434
C2  - pmc:PMC3140691
DO  - DOI:10.3389/fnins.2011.00089
UR  - https://pub.dzne.de/record/135985
ER  -