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@ARTICLE{Babu:135985,
      author       = {Babu, Harish and Claasen, Jan-Hendrik and Kannan, Suresh
                      and Rünker, Annette E and Palmer, Theo and Kempermann,
                      Gerd},
      title        = {{A} protocol for isolation and enriched monolayer
                      cultivation of neural precursor cells from mouse dentate
                      gyrus.},
      journal      = {Frontiers in neuroscience},
      volume       = {5},
      issn         = {1662-4548},
      address      = {Lausanne},
      publisher    = {Frontiers Research Foundation},
      reportid     = {DZNE-2020-02307},
      pages        = {89},
      year         = {2011},
      abstract     = {In vitro assays are valuable tools to study the
                      characteristics of adult neural precursor cells under
                      controlled conditions with a defined set of parameters. We
                      here present a detailed protocol based on our previous
                      original publication (Babu et al., 2007) to isolate neural
                      precursor cells from the hippocampus of adult mice and
                      maintain and propagate them as adherent monolayer cultures.
                      The strategy is based on the use of Percoll density gradient
                      centrifugation to enrich precursor cells from the
                      micro-dissected dentate gyrus. Based on the expression of
                      Nestin and Sox2, a culture-purity of more than $98\%$ can be
                      achieved. The cultures are expanded under serum-free
                      conditions in Neurobasal A medium with addition of the
                      mitogens Epidermal growth factor and Fibroblast growth
                      factor 2 as well as the supplements Glutamax-1 and B27.
                      Under differentiation conditions, the precursor cells
                      reliably generate approximately $30\%$ neurons with
                      appropriate morphological, molecular, and
                      electrophysiological characteristics that might reflect
                      granule cell properties as their in vivo counterpart. We
                      also highlight potential modifications to the protocol.},
      cin          = {AG Kempermann 1},
      ddc          = {610},
      cid          = {I:(DE-2719)1710001},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:21811434},
      pmc          = {pmc:PMC3140691},
      doi          = {10.3389/fnins.2011.00089},
      url          = {https://pub.dzne.de/record/135985},
}