TY  - JOUR
AU  - Schumacher, Miria C
AU  - Resenberger, Ulrike
AU  - Seidel, Ralf P
AU  - Becker, Christian F W
AU  - Winklhofer, Konstanze F
AU  - Oesterhelt, Dieter
AU  - Tatzelt, Jörg
AU  - Engelhard, Martin
TI  - Synthesis of a GPI anchor module suitable for protein post-translational modification.
JO  - Biopolymers
VL  - 94
IS  - 4
SN  - 0006-3525
CY  - New York, NY
PB  - Wiley76466
M1  - DZNE-2020-02387
SP  - 457-464
PY  - 2010
AB  - Eukaryotic cell surface proteins are often modified by a glycosylphosphatidylinositol (GPI) anchor. More than 200 of these post-translationally altered proteins are presently known, a prominent example being the prion protein (PrP). Although the significance of the GPI anchor is well recognized, efforts to study its function are hampered due to its complex chemical nature, which combines hydrophilic glycosyl chains with hydrophobic lipid moieties. Here we describe a general method for the synthesis of a GPI-anchored peptide containing an N-terminal Cys. This module can be employed for the production of proteins containing a natural GPI anchor using expressed protein ligation.
KW  - Glycosylphosphatidylinositols: chemical synthesis
KW  - Glycosylphosphatidylinositols: chemistry
KW  - Glycosylphosphatidylinositols: genetics
KW  - Humans
KW  - Peptides: chemical synthesis
KW  - Peptides: chemistry
KW  - Peptides: genetics
KW  - Prions: biosynthesis
KW  - Prions: chemical synthesis
KW  - Prions: chemistry
KW  - Prions: genetics
KW  - Protein Processing, Post-Translational
KW  - Saccharomyces cerevisiae: genetics
KW  - Saccharomyces cerevisiae: metabolism
KW  - Glycosylphosphatidylinositols (NLM Chemicals)
KW  - Peptides (NLM Chemicals)
KW  - Prions (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:20593476
DO  - DOI:10.1002/bip.21380
UR  - https://pub.dzne.de/record/136065
ER  -