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@ARTICLE{Schumacher:136065,
author = {Schumacher, Miria C and Resenberger, Ulrike and Seidel,
Ralf P and Becker, Christian F W and Winklhofer, Konstanze F
and Oesterhelt, Dieter and Tatzelt, Jörg and Engelhard,
Martin},
title = {{S}ynthesis of a {GPI} anchor module suitable for protein
post-translational modification.},
journal = {Biopolymers},
volume = {94},
number = {4},
issn = {0006-3525},
address = {New York, NY},
publisher = {Wiley76466},
reportid = {DZNE-2020-02387},
pages = {457-464},
year = {2010},
abstract = {Eukaryotic cell surface proteins are often modified by a
glycosylphosphatidylinositol (GPI) anchor. More than 200 of
these post-translationally altered proteins are presently
known, a prominent example being the prion protein (PrP).
Although the significance of the GPI anchor is well
recognized, efforts to study its function are hampered due
to its complex chemical nature, which combines hydrophilic
glycosyl chains with hydrophobic lipid moieties. Here we
describe a general method for the synthesis of a
GPI-anchored peptide containing an N-terminal Cys. This
module can be employed for the production of proteins
containing a natural GPI anchor using expressed protein
ligation.},
keywords = {Glycosylphosphatidylinositols: chemical synthesis /
Glycosylphosphatidylinositols: chemistry /
Glycosylphosphatidylinositols: genetics / Humans / Peptides:
chemical synthesis / Peptides: chemistry / Peptides:
genetics / Prions: biosynthesis / Prions: chemical synthesis
/ Prions: chemistry / Prions: genetics / Protein Processing,
Post-Translational / Saccharomyces cerevisiae: genetics /
Saccharomyces cerevisiae: metabolism /
Glycosylphosphatidylinositols (NLM Chemicals) / Peptides
(NLM Chemicals) / Prions (NLM Chemicals)},
cin = {Ext AG Tatzelt / AG Winklhofer},
ddc = {540},
cid = {I:(DE-2719)5000053 / I:(DE-2719)5000047},
pnm = {341 - Molecular Signaling (POF3-341) / 342 - Disease
Mechanisms and Model Systems (POF3-342)},
pid = {G:(DE-HGF)POF3-341 / G:(DE-HGF)POF3-342},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20593476},
doi = {10.1002/bip.21380},
url = {https://pub.dzne.de/record/136065},
}