%0 Journal Article
%A Cox, Brian J
%A Vollmer, Marion
%A Tamplin, Owen
%A Lu, Mei
%A Biechele, Steffen
%A Gertsenstein, Marina
%A van Campenhout, Claude
%A Floss, Thomas
%A Kühn, Ralf
%A Wurst, Wolfgang
%A Lickert, Heiko
%A Rossant, Janet
%T Phenotypic annotation of the mouse X chromosome.
%J Genome research
%V 20
%N 8
%@ 1088-9051
%C Cold Spring Harbor, NY
%I Laboratory Press
%M DZNE-2020-02406
%P 1154-1164
%D 2010
%X Mutational screens are an effective means used in the functional annotation of a genome. We present a method for a mutational screen of the mouse X chromosome using gene trap technologies. This method has the potential to screen all of the genes on the X chromosome without establishing mutant animals, as all gene-trapped embryonic stem (ES) cell lines are hemizygous null for mutations on the X chromosome. Based on this method, embryonic morphological phenotypes and expression patterns for 58 genes were assessed, approximately 10
%K Acyltransferases
%K Animals
%K Base Sequence
%K Chromosomes, Human, X: genetics
%K DNA Mutational Analysis: methods
%K Genes, Lethal
%K Genes, X-Linked
%K Genetic Diseases, X-Linked: genetics
%K Genetic Predisposition to Disease
%K Genetic Testing: methods
%K Humans
%K Membrane Proteins: genetics
%K Mental Retardation, X-Linked: genetics
%K Mice
%K Molecular Sequence Annotation
%K Molecular Sequence Data
%K Phenotype
%K Proto-Oncogene Proteins: genetics
%K Repressor Proteins: genetics
%K X Chromosome: genetics
%K BCOR protein, human (NLM Chemicals)
%K Membrane Proteins (NLM Chemicals)
%K Proto-Oncogene Proteins (NLM Chemicals)
%K Repressor Proteins (NLM Chemicals)
%K Acyltransferases (NLM Chemicals)
%K PORCN protein, human (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:20548051
%2 pmc:PMC2909578
%R 10.1101/gr.105106.110
%U https://pub.dzne.de/record/136084