Home > Publications Database > The Nicastrin ectodomain adopts a highly thermostable structure. > print

001     136321
005     20240613094805.0
024 7 _ |a 10.1515/BC.2011.169
|2 doi
024 7 _ |a pmid:21848507
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024 7 _ |a 1431-6730
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024 7 _ |a 1437-4315
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037 _ _ |a DZNE-2020-02643
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Fluhrer, Regina
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245 _ _ |a The Nicastrin ectodomain adopts a highly thermostable structure.
260 _ _ |a Berlin [u.a.]
|c 2011
|b de Gruyter
264 _ 1 |3 print
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|b Walter de Gruyter GmbH
|c 2011-11-01
336 7 _ |a article
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336 7 _ |a Output Types/Journal article
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336 7 _ |a ARTICLE
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336 7 _ |a Journal Article
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520 _ _ |a Nicastrin is a type I transmembrane glycoprotein, which is part of the high molecular weight γ-secretase complex. γ-Secretase is one of the key players associated with the generation of Alzheimer's disease pathology, since it liberates the neurotoxic amyloid β-peptide. Four proteins Nicastrin, anterior pharynx-defective-1 (Aph-1), presenilin enhancer-2 (Pen-2) and Presenilin are essential to form the active γ-secretase complex. Recently it has been shown, that Nicastrin has a key function in stabilizing the mature γ-secretase complex and may also be involved in substrate recognition. So far no structural data for the Nicastrin ectodomain or any other γ-secretase component are available. We therefore used Circular Dichroism (CD) spectroscopy to demonstrate that Nicastrin, similar to its homologues, the Streptomyces griseus aminopeptidase (SGAP) and the transferrin receptor (TfR), adopts a thermostable secondary structure. Furthermore, the Nicastrin ectodomain has an exceptionally high propensity to refold after thermal denaturation. These findings provide evidence to further support the hypothesis that Nicastrin may share evolutionary conserved properties with the aminopeptidase and the transferrin receptor family.
536 _ _ |a 342 - Disease Mechanisms and Model Systems (POF3-342)
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650 _ 7 |a Membrane Glycoproteins
|2 NLM Chemicals
650 _ 7 |a Receptors, Transferrin
|2 NLM Chemicals
650 _ 7 |a nicastrin protein
|2 NLM Chemicals
650 _ 7 |a Amyloid Precursor Protein Secretases
|0 EC 3.4.-
|2 NLM Chemicals
650 _ 7 |a Aminopeptidases
|0 EC 3.4.11.-
|2 NLM Chemicals
650 _ 2 |a Alzheimer Disease: metabolism
|2 MeSH
650 _ 2 |a Aminopeptidases: chemistry
|2 MeSH
650 _ 2 |a Amyloid Precursor Protein Secretases: chemistry
|2 MeSH
650 _ 2 |a Cell Line
|2 MeSH
650 _ 2 |a Circular Dichroism
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Membrane Glycoproteins: chemistry
|2 MeSH
650 _ 2 |a Protein Refolding
|2 MeSH
650 _ 2 |a Protein Stability
|2 MeSH
650 _ 2 |a Protein Structure, Secondary
|2 MeSH
650 _ 2 |a Protein Structure, Tertiary
|2 MeSH
650 _ 2 |a Receptors, Transferrin: chemistry
|2 MeSH
650 _ 2 |a Streptomyces griseus: enzymology
|2 MeSH
650 _ 2 |a Temperature
|2 MeSH
700 1 _ |a Kamp, Frits
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Grammer, Gudula
|b 2
700 1 _ |a Nuscher, Brigitte
|0 P:(DE-HGF)0
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700 1 _ |a Steiner, Harald
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700 1 _ |a Beyer, Klaus
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700 1 _ |a Haass, Christian
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773 1 8 |a 10.1515/bc.2011.169
|b : Walter de Gruyter GmbH, 2011-11-01
|n 11
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|t Biological Chemistry
|v 392
|y 2011
|x 1437-4315
773 _ _ |a 10.1515/BC.2011.169
|g Vol. 392, no. 11
|0 PERI:(DE-600)1466062-3
|n 11
|q 392:11
|p 995–1001
|t Biological chemistry
|v 392
|y 2011
|x 1437-4315
856 4 _ |u https://pub.dzne.de/record/136321/files/DZNE-2020-02643_Restricted.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.273.6.3205
|2 Crossref
|o 10.1074/jbc.273.6.3205
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.C300435200
|2 Crossref
|o 10.1074/jbc.C300435200
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M803040200
|2 Crossref
|o 10.1074/jbc.M803040200
999 C 5 |9 -- missing cx lookup --
|a 10.1038/34910
|2 Crossref
|o 10.1038/34910
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M109.038737
|2 Crossref
|o 10.1074/jbc.M109.038737
999 C 5 |9 -- missing cx lookup --
|a 10.1073/pnas.132277899
|2 Crossref
|o 10.1073/pnas.132277899
999 C 5 |9 -- missing cx lookup --
|a 10.1038/ncb960
|2 Crossref
|o 10.1038/ncb960
999 C 5 |9 -- missing cx lookup --
|a 10.1016/S0968-0004(01)01789-3
|2 Crossref
|o 10.1016/S0968-0004(01)01789-3
999 C 5 |9 -- missing cx lookup --
|a 10.1016/S1534-5807(02)00189-2
|2 Crossref
|o 10.1016/S1534-5807(02)00189-2
999 C 5 |9 -- missing cx lookup --
|a 10.1523/JNEUROSCI.1443-10.2010
|2 Crossref
|o 10.1523/JNEUROSCI.1443-10.2010
999 C 5 |y 2000
|2 Crossref
|o Goutte C. 2000
999 C 5 |9 -- missing cx lookup --
|a 10.1038/nrm2101
|2 Crossref
|o 10.1038/nrm2101
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M109.055061
|2 Crossref
|o 10.1074/jbc.M109.055061
999 C 5 |9 -- missing cx lookup --
|a 10.1038/35017105
|2 Crossref
|o 10.1038/35017105
999 C 5 |9 -- missing cx lookup --
|a 10.1242/jcs.00292
|2 Crossref
|o 10.1242/jcs.00292
999 C 5 |9 -- missing cx lookup --
|a 10.1083/jcb.200201123
|2 Crossref
|o 10.1083/jcb.200201123
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M204446200
|2 Crossref
|o 10.1074/jbc.M204446200
999 C 5 |9 -- missing cx lookup --
|a 10.1073/pnas.1037392100
|2 Crossref
|o 10.1073/pnas.1037392100
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M303941200
|2 Crossref
|o 10.1074/jbc.M303941200
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M208164200
|2 Crossref
|o 10.1074/jbc.M208164200
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.C200148200
|2 Crossref
|o 10.1074/jbc.C200148200
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M807485200
|2 Crossref
|o 10.1074/jbc.M807485200
999 C 5 |9 -- missing cx lookup --
|a 10.1006/abbi.2000.2177
|2 Crossref
|o 10.1006/abbi.2000.2177
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M401789200
|2 Crossref
|o 10.1074/jbc.M401789200
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.272.39.24536
|2 Crossref
|o 10.1074/jbc.272.39.24536
999 C 5 |9 -- missing cx lookup --
|a 10.1016/S0304-4157(96)00014-7
|2 Crossref
|o 10.1016/S0304-4157(96)00014-7
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.cell.2005.05.022
|2 Crossref
|o 10.1016/j.cell.2005.05.022
999 C 5 |9 -- missing cx lookup --
|a 10.1073/pnas.0801340105
|2 Crossref
|o 10.1073/pnas.0801340105
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.C300095200
|2 Crossref
|o 10.1074/jbc.C300095200
999 C 5 |9 -- missing cx lookup --
|a 10.1111/j.1471-4159.2004.02447.x
|2 Crossref
|o 10.1111/j.1471-4159.2004.02447.x
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.274.40.28669
|2 Crossref
|o 10.1074/jbc.274.40.28669
999 C 5 |9 -- missing cx lookup --
|a 10.1038/35041097
|2 Crossref
|o 10.1038/35041097
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.C200469200
|2 Crossref
|o 10.1074/jbc.C200469200
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.R800010200
|2 Crossref
|o 10.1074/jbc.R800010200
999 C 5 |9 -- missing cx lookup --
|a 10.1038/nature01506
|2 Crossref
|o 10.1038/nature01506
999 C 5 |9 -- missing cx lookup --
|a 10.1016/S0896-6273(00)80291-3
|2 Crossref
|o 10.1016/S0896-6273(00)80291-3
999 C 5 |9 -- missing cx lookup --
|a 10.1016/S0014-5793(02)02802-8
|2 Crossref
|o 10.1016/S0014-5793(02)02802-8
999 C 5 |y 1973
|2 Crossref
|o Vosbeck K.D. 1973
999 C 5 |9 -- missing cx lookup --
|a 10.1038/19077
|2 Crossref
|o 10.1038/19077
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M110871200
|2 Crossref
|o 10.1074/jbc.M110871200
999 C 5 |9 -- missing cx lookup --
|a 10.1038/35024009
|2 Crossref
|o 10.1038/35024009
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.C100410200
|2 Crossref
|o 10.1074/jbc.C100410200
999 C 5 |9 -- missing cx lookup --
|a 10.1038/35017108
|2 Crossref
|o 10.1038/35017108
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M409467200
|2 Crossref
|o 10.1074/jbc.M409467200
999 C 5 |9 -- missing cx lookup --
|a 10.1523/JNEUROSCI.3826-09.2010
|2 Crossref
|o 10.1523/JNEUROSCI.3826-09.2010

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