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| 001 | 136446 | ||
| 005 | 20240614160202.0 | ||
| 024 | 7 | _ | |a 10.1016/j.bios.2011.12.026 |2 doi |
| 024 | 7 | _ | |a pmid:22221799 |2 pmid |
| 024 | 7 | _ | |a 0956-5663 |2 ISSN |
| 024 | 7 | _ | |a 1873-4235 |2 ISSN |
| 037 | _ | _ | |a DZNE-2020-02768 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Jahnke, Heinz-Georg |b 0 |
| 245 | _ | _ | |a Impedance spectroscopy based measurement system for quantitative and label-free real-time monitoring of tauopathy in hippocampal slice cultures. |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2012 |b Elsevier Science |
| 264 | _ | 1 | |3 print |2 Crossref |b Elsevier BV |c 2012-02-01 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1718373666_613 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Alzheimer's disease (AD) and other tauopathies comprise death of cell bodies, synapses and neurites but there is surprising little knowledge of the temporal sequence and the causal relationships among these events. Here, we present a novel biosensoric approach to monitor retrograde neurite degeneration before cell death occurs. We induced tau hyperphosphorylation in organotypic hippocampal slice cultures (OHSC) and applied marker-independent real-time electrical impedance spectroscopy (EIS) for cellular real-time pathology monitoring. Using this approach, we were able to define two distinct phases of neurite degeneration, first a rapid swelling of axonal processes that manifests itself in relative impedance above control levels followed by a slower phase of collapse and subsequent fragmentation indicated by decreased relative impedance below control levels. Initial axon swelling is strictly dose-dependent and swelling intensity correlates with second phase impedance decrease implicating a causative link between both degenerative mechanisms. Moreover, suppressing tau hyperphosphorylation by kinase inhibition nearly prevented both phases of axon degeneration. Our findings demonstrate that the temporal sequence of tau-triggered neurite degeneration can be directly visualized by EIS-based, non-invasive and label-free monitoring. We therefore suggest this approach as a powerful extension of high content applications to study mechanisms of neurite degeneration and to exploit therapeutic options against AD and tau-related disorders. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 542 | _ | _ | |i 2012-02-01 |2 Crossref |u https://www.elsevier.com/tdm/userlicense/1.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Carbazoles |2 NLM Chemicals |
| 650 | _ | 7 | |a Enzyme Inhibitors |2 NLM Chemicals |
| 650 | _ | 7 | |a SRN 003-556 |2 NLM Chemicals |
| 650 | _ | 7 | |a tau Proteins |2 NLM Chemicals |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Biosensing Techniques: instrumentation |2 MeSH |
| 650 | _ | 2 | |a Carbazoles: pharmacology |2 MeSH |
| 650 | _ | 2 | |a Cells, Cultured |2 MeSH |
| 650 | _ | 2 | |a Dielectric Spectroscopy: instrumentation |2 MeSH |
| 650 | _ | 2 | |a Enzyme Inhibitors: pharmacology |2 MeSH |
| 650 | _ | 2 | |a Equipment Design |2 MeSH |
| 650 | _ | 2 | |a Hippocampus: cytology |2 MeSH |
| 650 | _ | 2 | |a Neurites: drug effects |2 MeSH |
| 650 | _ | 2 | |a Neurites: pathology |2 MeSH |
| 650 | _ | 2 | |a Rats |2 MeSH |
| 650 | _ | 2 | |a Rats, Sprague-Dawley |2 MeSH |
| 650 | _ | 2 | |a Tauopathies: drug therapy |2 MeSH |
| 650 | _ | 2 | |a Tauopathies: pathology |2 MeSH |
| 650 | _ | 2 | |a tau Proteins: antagonists & inhibitors |2 MeSH |
| 700 | 1 | _ | |a Braesigk, Annett |b 1 |
| 700 | 1 | _ | |a Mack, Till G A |0 P:(DE-2719)9000424 |b 2 |u dzne |
| 700 | 1 | _ | |a Pönick, Sarah |b 3 |
| 700 | 1 | _ | |a Striggow, Frank |0 P:(DE-2719)9000420 |b 4 |u dzne |
| 700 | 1 | _ | |a Robitzki, Andrea A |0 P:(DE-HGF)0 |b 5 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1016/j.bios.2011.12.026 |b : Elsevier BV, 2012-02-01 |n 1 |p 250-258 |3 journal-article |2 Crossref |t Biosensors and Bioelectronics |v 32 |y 2012 |x 0956-5663 |
| 773 | _ | _ | |a 10.1016/j.bios.2011.12.026 |g Vol. 32, no. 1, p. 250 - 258 |0 PERI:(DE-600)1496379-6 |n 1 |q 32:1<250 - 258 |p 250-258 |t Biosensors and bioelectronics |v 32 |y 2012 |x 0956-5663 |
| 856 | 4 | _ | |u https://pub.dzne.de/record/136446/files/DZNE-2020-02768_Restricted.pdf |
| 856 | 4 | _ | |u https://pub.dzne.de/record/136446/files/DZNE-2020-02768_Restricted.pdf?subformat=pdfa |x pdfa |
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| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 4 |6 P:(DE-2719)9000420 |
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