%0 Journal Article
%A Röhnert, Peter
%A Schmidt, Werner
%A Emmerlich, Patrick
%A Goihl, Alexander
%A Wrenger, Sabine
%A Bank, Ute
%A Nordhoff, Karsten
%A Täger, Michael
%A Ansorge, Siegfried
%A Reinhold, Dirk
%A Striggow, Frank
%T Dipeptidyl peptidase IV, aminopeptidase N and DPIV/APN-like proteases in cerebral ischemia.
%J Journal of neuroinflammation
%V 9
%N 1
%@ 1742-2094
%C London
%I BioMed Central
%M DZNE-2020-02850
%P 557
%D 2012
%X Cerebral inflammation is a hallmark of neuronal degeneration. Dipeptidyl peptidase IV, aminopeptidase N as well as the dipeptidyl peptidases II, 8 and 9 and cytosolic alanyl-aminopeptidase are involved in the regulation of autoimmunity and inflammation. We studied the expression, localisation and activity patterns of these proteases after endothelin-induced occlusion of the middle cerebral artery in rats, a model of transient and unilateral cerebral ischemia.Male Sprague-Dawley rats were used. RT-PCR, immunohistochemistry and protease activity assays were performed at different time points, lasting from 2 h to 7 days after cerebral ischemia. The effect of protease inhibitors on ischemia-dependent infarct volumes was quantified 7 days post middle cerebral artery occlusion. Statistical analysis was conducted using the t-test.Qualitative RT-PCR revealed these proteases in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N were up-regulated ipsilaterally from 6 h to 7 days post ischemia, whereas dipeptidyl peptidase 9 and cytosolic alanyl-aminopeptidase were transiently down-regulated at day 3. Dipeptidyl peptidase 8 and aminopeptidase N immunoreactivities were detected in cortical neurons of the contralateral hemisphere. At the same time point, dipeptidyl peptidase IV, 8 and aminopeptidase N were identified in activated microglia and macrophages in the ipsilateral cortex. Seven days post artery occlusion, dipeptidyl peptidase IV immunoreactivity was found in the perikarya of surviving cortical neurons of the ipsilateral hemisphere, whereas their nuclei were dipeptidyl peptidase 8- and amino peptidase N-positive. At the same time point, dipeptidyl peptidase IV, 8 and aminopeptidase N were targeted in astroglial cells. Total dipeptidyl peptidase IV, 8 and 9 activities remained constant in both hemispheres until day 3 post experimental ischemia, but were increased (+165
%K Animals
%K Brain Ischemia: complications
%K Brain Ischemia: drug therapy
%K Brain Ischemia: enzymology
%K CD13 Antigens: genetics
%K CD13 Antigens: metabolism
%K Cerebral Infarction: enzymology
%K Cerebral Infarction: etiology
%K Dipeptidyl Peptidase 4: genetics
%K Dipeptidyl Peptidase 4: metabolism
%K Dipeptidyl-Peptidases and Tripeptidyl-Peptidases: genetics
%K Dipeptidyl-Peptidases and Tripeptidyl-Peptidases: metabolism
%K Disease Models, Animal
%K Enzyme Inhibitors: therapeutic use
%K Functional Laterality
%K Gene Expression Regulation, Enzymologic: physiology
%K Glial Fibrillary Acidic Protein: metabolism
%K Glycosphingolipids: therapeutic use
%K Male
%K Phosphopyruvate Hydratase: metabolism
%K RNA, Messenger: metabolism
%K Rats
%K Rats, Sprague-Dawley
%K Time Factors
%K Enzyme Inhibitors (NLM Chemicals)
%K Glial Fibrillary Acidic Protein (NLM Chemicals)
%K Glycosphingolipids (NLM Chemicals)
%K RNA, Messenger (NLM Chemicals)
%K inositolphosphorylceramide (NLM Chemicals)
%K CD13 Antigens (NLM Chemicals)
%K Dipeptidyl-Peptidases and Tripeptidyl-Peptidases (NLM Chemicals)
%K Dpp8 protein, rat (NLM Chemicals)
%K Dipeptidyl Peptidase 4 (NLM Chemicals)
%K Phosphopyruvate Hydratase (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:22373413
%2 pmc:PMC3359160
%R 10.1186/1742-2094-9-44
%U https://pub.dzne.de/record/136528