TY  - JOUR
AU  - Röhnert, Peter
AU  - Schmidt, Werner
AU  - Emmerlich, Patrick
AU  - Goihl, Alexander
AU  - Wrenger, Sabine
AU  - Bank, Ute
AU  - Nordhoff, Karsten
AU  - Täger, Michael
AU  - Ansorge, Siegfried
AU  - Reinhold, Dirk
AU  - Striggow, Frank
TI  - Dipeptidyl peptidase IV, aminopeptidase N and DPIV/APN-like proteases in cerebral ischemia.
JO  - Journal of neuroinflammation
VL  - 9
IS  - 1
SN  - 1742-2094
CY  - London
PB  - BioMed Central
M1  - DZNE-2020-02850
SP  - 557
PY  - 2012
AB  - Cerebral inflammation is a hallmark of neuronal degeneration. Dipeptidyl peptidase IV, aminopeptidase N as well as the dipeptidyl peptidases II, 8 and 9 and cytosolic alanyl-aminopeptidase are involved in the regulation of autoimmunity and inflammation. We studied the expression, localisation and activity patterns of these proteases after endothelin-induced occlusion of the middle cerebral artery in rats, a model of transient and unilateral cerebral ischemia.Male Sprague-Dawley rats were used. RT-PCR, immunohistochemistry and protease activity assays were performed at different time points, lasting from 2 h to 7 days after cerebral ischemia. The effect of protease inhibitors on ischemia-dependent infarct volumes was quantified 7 days post middle cerebral artery occlusion. Statistical analysis was conducted using the t-test.Qualitative RT-PCR revealed these proteases in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N were up-regulated ipsilaterally from 6 h to 7 days post ischemia, whereas dipeptidyl peptidase 9 and cytosolic alanyl-aminopeptidase were transiently down-regulated at day 3. Dipeptidyl peptidase 8 and aminopeptidase N immunoreactivities were detected in cortical neurons of the contralateral hemisphere. At the same time point, dipeptidyl peptidase IV, 8 and aminopeptidase N were identified in activated microglia and macrophages in the ipsilateral cortex. Seven days post artery occlusion, dipeptidyl peptidase IV immunoreactivity was found in the perikarya of surviving cortical neurons of the ipsilateral hemisphere, whereas their nuclei were dipeptidyl peptidase 8- and amino peptidase N-positive. At the same time point, dipeptidyl peptidase IV, 8 and aminopeptidase N were targeted in astroglial cells. Total dipeptidyl peptidase IV, 8 and 9 activities remained constant in both hemispheres until day 3 post experimental ischemia, but were increased (+165
KW  - Animals
KW  - Brain Ischemia: complications
KW  - Brain Ischemia: drug therapy
KW  - Brain Ischemia: enzymology
KW  - CD13 Antigens: genetics
KW  - CD13 Antigens: metabolism
KW  - Cerebral Infarction: enzymology
KW  - Cerebral Infarction: etiology
KW  - Dipeptidyl Peptidase 4: genetics
KW  - Dipeptidyl Peptidase 4: metabolism
KW  - Dipeptidyl-Peptidases and Tripeptidyl-Peptidases: genetics
KW  - Dipeptidyl-Peptidases and Tripeptidyl-Peptidases: metabolism
KW  - Disease Models, Animal
KW  - Enzyme Inhibitors: therapeutic use
KW  - Functional Laterality
KW  - Gene Expression Regulation, Enzymologic: physiology
KW  - Glial Fibrillary Acidic Protein: metabolism
KW  - Glycosphingolipids: therapeutic use
KW  - Male
KW  - Phosphopyruvate Hydratase: metabolism
KW  - RNA, Messenger: metabolism
KW  - Rats
KW  - Rats, Sprague-Dawley
KW  - Time Factors
KW  - Enzyme Inhibitors (NLM Chemicals)
KW  - Glial Fibrillary Acidic Protein (NLM Chemicals)
KW  - Glycosphingolipids (NLM Chemicals)
KW  - RNA, Messenger (NLM Chemicals)
KW  - inositolphosphorylceramide (NLM Chemicals)
KW  - CD13 Antigens (NLM Chemicals)
KW  - Dipeptidyl-Peptidases and Tripeptidyl-Peptidases (NLM Chemicals)
KW  - Dpp8 protein, rat (NLM Chemicals)
KW  - Dipeptidyl Peptidase 4 (NLM Chemicals)
KW  - Phosphopyruvate Hydratase (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22373413
C2  - pmc:PMC3359160
DO  - DOI:10.1186/1742-2094-9-44
UR  - https://pub.dzne.de/record/136528
ER  -