TY  - JOUR
AU  - Flynn, Kevin C
AU  - Hellal, Farida
AU  - Neukirchen, Dorothee
AU  - Jacob, Sonja
AU  - Tahirovic, Sabina
AU  - Dupraz, Sebastian
AU  - Stern, Sina
AU  - Garvalov, Boyan K
AU  - Gurniak, Christine
AU  - Shaw, Alisa E
AU  - Meyn, Liane
AU  - Wedlich-Söldner, Roland
AU  - Bamburg, James R
AU  - Small, J Victor
AU  - Witke, Walter
AU  - Bradke, Frank
TI  - ADF/cofilin-mediated actin retrograde flow directs neurite formation in the developing brain.
JO  - Neuron
VL  - 76
IS  - 6
SN  - 0896-6273
CY  - New York, NY
PB  - Elsevier
M1  - DZNE-2020-03077
SP  - 1091-1107
PY  - 2012
AB  - Neurites are the characteristic structural element of neurons that will initiate brain connectivity and elaborate information. Early in development, neurons are spherical cells but this symmetry is broken through the initial formation of neurites. This fundamental step is thought to rely on actin and microtubule dynamics. However, it is unclear which aspects of the complex actin behavior control neuritogenesis and which molecular mechanisms are involved. Here, we demonstrate that augmented actin retrograde flow and protrusion dynamics facilitate neurite formation. Our data indicate that a single family of actin regulatory proteins, ADF/Cofilin, provides the required control of actin retrograde flow and dynamics to form neurites. In particular, the F-actin severing activity of ADF/Cofilin organizes space for the protrusion and bundling of microtubules, the backbone of neurites. Our data reveal how ADF/Cofilin organizes the cytoskeleton to drive actin retrograde flow and thus break the spherical shape of neurons.
KW  - Actin Depolymerizing Factors: physiology
KW  - Actins: metabolism
KW  - Animals
KW  - Biological Transport
KW  - Cell Growth Processes: physiology
KW  - Cell Shape: physiology
KW  - Cells, Cultured
KW  - Cerebral Cortex: cytology
KW  - Cerebral Cortex: embryology
KW  - Destrin: physiology
KW  - Growth Cones: metabolism
KW  - Hippocampus: cytology
KW  - Hippocampus: embryology
KW  - In Vitro Techniques
KW  - Mice
KW  - Mice, Knockout
KW  - Microtubules: physiology
KW  - Neurites: metabolism
KW  - Neurogenesis: physiology
KW  - Actin Depolymerizing Factors (NLM Chemicals)
KW  - Actins (NLM Chemicals)
KW  - Destrin (NLM Chemicals)
KW  - Dstn protein, mouse (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:23259946
DO  - DOI:10.1016/j.neuron.2012.09.038
UR  - https://pub.dzne.de/record/136755
ER  -