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@ARTICLE{Flynn:136755,
author = {Flynn, Kevin C and Hellal, Farida and Neukirchen, Dorothee
and Jacob, Sonja and Tahirovic, Sabina and Dupraz, Sebastian
and Stern, Sina and Garvalov, Boyan K and Gurniak, Christine
and Shaw, Alisa E and Meyn, Liane and Wedlich-Söldner,
Roland and Bamburg, James R and Small, J Victor and Witke,
Walter and Bradke, Frank},
title = {{ADF}/cofilin-mediated actin retrograde flow directs
neurite formation in the developing brain.},
journal = {Neuron},
volume = {76},
number = {6},
issn = {0896-6273},
address = {New York, NY},
publisher = {Elsevier},
reportid = {DZNE-2020-03077},
pages = {1091-1107},
year = {2012},
abstract = {Neurites are the characteristic structural element of
neurons that will initiate brain connectivity and elaborate
information. Early in development, neurons are spherical
cells but this symmetry is broken through the initial
formation of neurites. This fundamental step is thought to
rely on actin and microtubule dynamics. However, it is
unclear which aspects of the complex actin behavior control
neuritogenesis and which molecular mechanisms are involved.
Here, we demonstrate that augmented actin retrograde flow
and protrusion dynamics facilitate neurite formation. Our
data indicate that a single family of actin regulatory
proteins, ADF/Cofilin, provides the required control of
actin retrograde flow and dynamics to form neurites. In
particular, the F-actin severing activity of ADF/Cofilin
organizes space for the protrusion and bundling of
microtubules, the backbone of neurites. Our data reveal how
ADF/Cofilin organizes the cytoskeleton to drive actin
retrograde flow and thus break the spherical shape of
neurons.},
keywords = {Actin Depolymerizing Factors: physiology / Actins:
metabolism / Animals / Biological Transport / Cell Growth
Processes: physiology / Cell Shape: physiology / Cells,
Cultured / Cerebral Cortex: cytology / Cerebral Cortex:
embryology / Destrin: physiology / Growth Cones: metabolism
/ Hippocampus: cytology / Hippocampus: embryology / In Vitro
Techniques / Mice / Mice, Knockout / Microtubules:
physiology / Neurites: metabolism / Neurogenesis: physiology
/ Actin Depolymerizing Factors (NLM Chemicals) / Actins (NLM
Chemicals) / Destrin (NLM Chemicals) / Dstn protein, mouse
(NLM Chemicals)},
cin = {AG Bradke / AG Tahirovic},
ddc = {610},
cid = {I:(DE-2719)1013002 / I:(DE-2719)1140003},
pnm = {341 - Molecular Signaling (POF3-341) / 342 - Disease
Mechanisms and Model Systems (POF3-342)},
pid = {G:(DE-HGF)POF3-341 / G:(DE-HGF)POF3-342},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:23259946},
doi = {10.1016/j.neuron.2012.09.038},
url = {https://pub.dzne.de/record/136755},
}