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@ARTICLE{Taverna:137076,
      author       = {Taverna, Mara and Straub, Tobias and Hampel, Harald and
                      Rujescu, Dan and Lichtenthaler, Stefan F},
      title        = {{A} new sandwich immunoassay for detection of the
                      α-secretase cleaved, soluble amyloid-β protein precursor
                      in cerebrospinal fluid and serum.},
      journal      = {Journal of Alzheimer's disease},
      volume       = {37},
      number       = {4},
      issn         = {1875-8908},
      address      = {Amsterdam},
      publisher    = {IOS Press},
      reportid     = {DZNE-2020-03398},
      pages        = {667-678},
      year         = {2013},
      abstract     = {Alzheimer's disease (AD) is the most common
                      neurodegenerative disorder. Frequently used diagnostic
                      biomarkers are amyloid-β42 (Aβ42), tau, and phospho-tau,
                      which are measured in cerebrospinal fluid (CSF), and allow a
                      reasonable, but not full, separation of AD patients and
                      controls. Besides Aβ42, additional proteolytic cleavage
                      products of the amyloid-β protein precursor (AβPP) have
                      been investigated as potential biomarkers. This includes the
                      α-secretase cleaved soluble AβPP ectodomain (sAβPPα).
                      However, some studies found a reduction of sAβPPα, whereas
                      other studies reported an increase of sAβPPα in the CSF of
                      AD patients. The divergent findings may result from the
                      detection of sAβPPα with antibodies, such as 6E10, which
                      do not exclusively detect sAβPPα, but also the alternative
                      β-secretase cleavage product sAβPPβ'. Here, we used the
                      sAβPPα-specific antibody 14D6 and developed an ELISA-like
                      sandwich immunoassay. The assay specifically detected
                      sAβPPα in cell culture supernatants, in human CSF and even
                      in serum, which is more readily accessible than CSF. The
                      assay was used to analyze sAβPPα levels in CSF and serum
                      of AD patients and controls. The assay detected a mild, but
                      significant increase in sAβPPα in the CSF of AD patients
                      compared to non-demented controls, while a mild reduction
                      was observed in serum. The 14D6 assay in CSF allowed a
                      better separation of AD patients from controls compared to
                      the 6E10 antibody. Taken together, the new assay is widely
                      applicable for specific sAβPPα measurement in culture
                      media, CSF, and serum.},
      keywords     = {Amino Acid Sequence / Amyloid Precursor Protein Secretases:
                      blood / Amyloid Precursor Protein Secretases: cerebrospinal
                      fluid / Amyloid Precursor Protein Secretases: genetics /
                      Amyloid beta-Protein Precursor: blood / Amyloid beta-Protein
                      Precursor: cerebrospinal fluid / Amyloid beta-Protein
                      Precursor: genetics / Biomarkers: blood / Biomarkers:
                      cerebrospinal fluid / Cell Line, Tumor / Enzyme-Linked
                      Immunosorbent Assay: trends / Female / Humans / Male /
                      Molecular Sequence Data / Registries / Amyloid beta-Protein
                      Precursor (NLM Chemicals) / Biomarkers (NLM Chemicals) /
                      Amyloid Precursor Protein Secretases (NLM Chemicals)},
      cin          = {AG Lichtenthaler},
      ddc          = {610},
      cid          = {I:(DE-2719)1110006},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:23948916},
      doi          = {10.3233/JAD-130509},
      url          = {https://pub.dzne.de/record/137076},
}