TY  - JOUR
AU  - Krähling, Anke Miriam
AU  - Alvarez, Luis
AU  - Debowski, Katharina
AU  - Van, Qui
AU  - Gunkel, Monika
AU  - Irsen, Stephan
AU  - Alamoudi, Ashraf
AU  - Strünker, Timo
AU  - Kremmer, Elisabeth
AU  - Krause, Eberhard
AU  - Voigt, Ingo
AU  - Wörtge, Simone
AU  - Waisman, Ari
AU  - Weyand, Ingo
AU  - Seifert, Reinhard
AU  - Kaupp, Ulrich Benjamin
AU  - Wachten, Dagmar
TI  - CRIS-a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending.
JO  - PLoS Genetics
VL  - 9
IS  - 12
SN  - 1553-7404
CY  - San Francisco, Calif.
PB  - Public Library of Science
M1  - DZNE-2020-03556
SP  - e1003960
PY  - 2013
AB  - The second messengers cAMP and cGMP activate their target proteins by binding to a conserved cyclic nucleotide-binding domain (CNBD). Here, we identify and characterize an entirely novel CNBD-containing protein called CRIS (cyclic nucleotide receptor involved in sperm function) that is unrelated to any of the other members of this protein family. CRIS is exclusively expressed in sperm precursor cells. Cris-deficient male mice are either infertile due to a lack of sperm resulting from spermatogenic arrest, or subfertile due to impaired sperm motility. The motility defect is caused by altered Ca(2+) regulation of flagellar beat asymmetry, leading to a beating pattern that is reminiscent of sperm hyperactivation. Our results suggest that CRIS interacts during spermiogenesis with Ca(2+)-regulated proteins that-in mature sperm-are involved in flagellar bending.
KW  - Animals
KW  - Calcium: metabolism
KW  - Carrier Proteins: genetics
KW  - Cyclic AMP: genetics
KW  - Cyclic AMP: metabolism
KW  - Cyclic GMP: metabolism
KW  - Flagella: genetics
KW  - Flagella: metabolism
KW  - Humans
KW  - Intracellular Signaling Peptides and Proteins
KW  - Male
KW  - Mice
KW  - Phosphorylation
KW  - Protein Binding: genetics
KW  - Signal Transduction: genetics
KW  - Sperm Motility: genetics
KW  - Spermatogenesis: genetics
KW  - Spermatozoa: metabolism
KW  - CRIS protein, mouse (NLM Chemicals)
KW  - Carrier Proteins (NLM Chemicals)
KW  - Intracellular Signaling Peptides and Proteins (NLM Chemicals)
KW  - Cyclic AMP (NLM Chemicals)
KW  - Cyclic GMP (NLM Chemicals)
KW  - Calcium (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:24339785
C2  - pmc:PMC3854790
DO  - DOI:10.1371/journal.pgen.1003960
UR  - https://pub.dzne.de/record/137234
ER  -