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@ARTICLE{Meier:137478,
author = {Meier, Florian and Giesert, Florian and Delic, Sabit and
Faus-Kessler, Theresa and Matheus, Friederike and Simeone,
Antonio and Hölter, Sabine M and Kühn, Ralf and
Vogt-Weisenhorn, Daniela and Wurst, Wolfgang and Prakash,
Nilima},
title = {{FGF}/{FGFR}2 signaling regulates the generation and
correct positioning of {B}ergmann glia cells in the
developing mouse cerebellum.},
journal = {PLOS ONE},
volume = {9},
number = {7},
issn = {1932-6203},
address = {San Francisco, California, US},
publisher = {PLOS},
reportid = {DZNE-2020-03800},
pages = {e101124},
year = {2014},
abstract = {The normal cellular organization and layering of the
vertebrate cerebellum is established during embryonic and
early postnatal development by the interplay of a complex
array of genetic and signaling pathways. Disruption of these
processes and of the proper layering of the cerebellum
usually leads to ataxic behaviors. Here, we analyzed the
relative contribution of Fibroblast growth factor receptor 2
(FGFR2)-mediated signaling to cerebellar development in
conditional Fgfr2 single mutant mice. We show that during
embryonic mouse development, Fgfr2 expression is higher in
the anterior cerebellar primordium and excluded from the
proliferative ventricular neuroepithelium. Consistent with
this finding, conditional Fgfr2 single mutant mice display
the most prominent defects in the anterior lobules of the
adult cerebellum. In this context, FGFR2-mediated signaling
is required for the proper generation of Bergmann glia cells
and the correct positioning of these cells within the
Purkinje cell layer, and for cell survival in the developing
cerebellar primordium. Using cerebellar microexplant
cultures treated with an FGFR agonist (FGF9) or antagonist
(SU5402), we also show that FGF9/FGFR-mediated signaling
inhibits the outward migration of radial glia and Bergmann
glia precursors and cells, and might thus act as a
positioning cue for these cells. Altogether, our findings
reveal the specific functions of the FGFR2-mediated
signaling pathway in the generation and positioning of
Bergmann glia cells during cerebellar development in the
mouse.},
keywords = {Animals / Cell Survival / Cerebellum: cytology /
Cerebellum: embryology / Cerebellum: metabolism / Fibroblast
Growth Factors: metabolism / Mice / Mice, Inbred C57BL /
Mice, Knockout / Neuroglia: cytology / Neuroglia: metabolism
/ Receptor, Fibroblast Growth Factor, Type 2: genetics /
Receptor, Fibroblast Growth Factor, Type 2: metabolism /
Signal Transduction / Fibroblast Growth Factors (NLM
Chemicals) / Fgfr2 protein, mouse (NLM Chemicals) /
Receptor, Fibroblast Growth Factor, Type 2 (NLM Chemicals)},
cin = {Ext HZM},
ddc = {610},
cid = {I:(DE-2719)5000050},
pnm = {341 - Molecular Signaling (POF3-341)},
pid = {G:(DE-HGF)POF3-341},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:24983448},
pmc = {pmc:PMC4077754},
doi = {10.1371/journal.pone.0101124},
url = {https://pub.dzne.de/record/137478},
}