TY  - JOUR
AU  - Müller, Janine
AU  - Ossig, Christiana
AU  - Greiner, Johannes F W
AU  - Hauser, Stefan
AU  - Fauser, Mareike
AU  - Widera, Darius
AU  - Kaltschmidt, Christian
AU  - Storch, Alexander
AU  - Kaltschmidt, Barbara
TI  - Intrastriatal transplantation of adult human neural crest-derived stem cells improves functional outcome in parkinsonian rats.
JO  - Stem cells translational medicine
VL  - 4
IS  - 1
SN  - 2157-6564
CY  - Durham
PB  - AlphaMed Press
M1  - DZNE-2020-04048
SP  - 31-43
PY  - 2015
AB  - Parkinson's disease (PD) is considered the second most frequent and one of the most severe neurodegenerative diseases, with dysfunctions of the motor system and with nonmotor symptoms such as depression and dementia. Compensation for the progressive loss of dopaminergic (DA) neurons during PD using current pharmacological treatment strategies is limited and remains challenging. Pluripotent stem cell-based regenerative medicine may offer a promising therapeutic alternative, although the medical application of human embryonic tissue and pluripotent stem cells is still a matter of ethical and practical debate. Addressing these challenges, the present study investigated the potential of adult human neural crest-derived stem cells derived from the inferior turbinate (ITSCs) transplanted into a parkinsonian rat model. Emphasizing their capability to give rise to nervous tissue, ITSCs isolated from the adult human nose efficiently differentiated into functional mature neurons in vitro. Additional successful dopaminergic differentiation of ITSCs was subsequently followed by their transplantation into a unilaterally lesioned 6-hydroxydopamine rat PD model. Transplantation of predifferentiated or undifferentiated ITSCs led to robust restoration of rotational behavior, accompanied by significant recovery of DA neurons within the substantia nigra. ITSCs were further shown to migrate extensively in loose streams primarily toward the posterior direction as far as to the midbrain region, at which point they were able to differentiate into DA neurons within the locus ceruleus. We demonstrate, for the first time, that adult human ITSCs are capable of functionally recovering a PD rat model.
KW  - Adult Stem Cells: cytology
KW  - Adult Stem Cells: transplantation
KW  - Animals
KW  - Cell Differentiation
KW  - Female
KW  - Heterografts
KW  - Humans
KW  - Immunohistochemistry
KW  - Neural Crest: cytology
KW  - Neural Crest: transplantation
KW  - Neural Stem Cells: cytology
KW  - Neural Stem Cells: transplantation
KW  - Parkinsonian Disorders: surgery
KW  - Rats
KW  - Rats, Wistar
KW  - Recovery of Function
KW  - Reverse Transcriptase Polymerase Chain Reaction
KW  - Stem Cell Transplantation: methods
LB  - PUB:(DE-HGF)16
C6  - pmid:25479965
C2  - pmc:PMC4275008
DO  - DOI:10.5966/sctm.2014-0078
UR  - https://pub.dzne.de/record/137726
ER  -