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@ARTICLE{Mller:137726,
author = {Müller, Janine and Ossig, Christiana and Greiner, Johannes
F W and Hauser, Stefan and Fauser, Mareike and Widera,
Darius and Kaltschmidt, Christian and Storch, Alexander and
Kaltschmidt, Barbara},
title = {{I}ntrastriatal transplantation of adult human neural
crest-derived stem cells improves functional outcome in
parkinsonian rats.},
journal = {Stem cells translational medicine},
volume = {4},
number = {1},
issn = {2157-6564},
address = {Durham},
publisher = {AlphaMed Press},
reportid = {DZNE-2020-04048},
pages = {31-43},
year = {2015},
abstract = {Parkinson's disease (PD) is considered the second most
frequent and one of the most severe neurodegenerative
diseases, with dysfunctions of the motor system and with
nonmotor symptoms such as depression and dementia.
Compensation for the progressive loss of dopaminergic (DA)
neurons during PD using current pharmacological treatment
strategies is limited and remains challenging. Pluripotent
stem cell-based regenerative medicine may offer a promising
therapeutic alternative, although the medical application of
human embryonic tissue and pluripotent stem cells is still a
matter of ethical and practical debate. Addressing these
challenges, the present study investigated the potential of
adult human neural crest-derived stem cells derived from the
inferior turbinate (ITSCs) transplanted into a parkinsonian
rat model. Emphasizing their capability to give rise to
nervous tissue, ITSCs isolated from the adult human nose
efficiently differentiated into functional mature neurons in
vitro. Additional successful dopaminergic differentiation of
ITSCs was subsequently followed by their transplantation
into a unilaterally lesioned 6-hydroxydopamine rat PD model.
Transplantation of predifferentiated or undifferentiated
ITSCs led to robust restoration of rotational behavior,
accompanied by significant recovery of DA neurons within the
substantia nigra. ITSCs were further shown to migrate
extensively in loose streams primarily toward the posterior
direction as far as to the midbrain region, at which point
they were able to differentiate into DA neurons within the
locus ceruleus. We demonstrate, for the first time, that
adult human ITSCs are capable of functionally recovering a
PD rat model.},
keywords = {Adult Stem Cells: cytology / Adult Stem Cells:
transplantation / Animals / Cell Differentiation / Female /
Heterografts / Humans / Immunohistochemistry / Neural Crest:
cytology / Neural Crest: transplantation / Neural Stem
Cells: cytology / Neural Stem Cells: transplantation /
Parkinsonian Disorders: surgery / Rats / Rats, Wistar /
Recovery of Function / Reverse Transcriptase Polymerase
Chain Reaction / Stem Cell Transplantation: methods},
cin = {AG Storch / AG Kühn},
ddc = {610},
cid = {I:(DE-2719)5000014 / I:(DE-2719)5000008},
pnm = {344 - Clinical and Health Care Research (POF3-344)},
pid = {G:(DE-HGF)POF3-344},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:25479965},
pmc = {pmc:PMC4275008},
doi = {10.5966/sctm.2014-0078},
url = {https://pub.dzne.de/record/137726},
}