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| 001 | 137945 | ||
| 005 | 20240321220338.0 | ||
| 024 | 7 | _ | |a 10.1371/journal.pone.0125418 |2 doi |
| 024 | 7 | _ | |a pmid:26017149 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC4446269 |2 pmc |
| 024 | 7 | _ | |a altmetric:4070118 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-04267 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Jährling, Nina |b 0 |
| 245 | _ | _ | |a Cerebral β-Amyloidosis in Mice Investigated by Ultramicroscopy. |
| 260 | _ | _ | |a San Francisco, California, US |c 2015 |b PLOS |
| 264 | _ | 1 | |3 online |2 Crossref |b Public Library of Science (PLoS) |c 2015-05-27 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1604653800_15311 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Alzheimer´s disease (AD) is the most common neurodegenerative disorder. AD neuropathology is characterized by intracellular neurofibrillary tangles and extracellular β-amyloid deposits in the brain. To elucidate the complexity of AD pathogenesis a variety of transgenic mouse models have been generated. An ideal imaging system for monitoring β-amyloid plaque deposition in the brain of these animals should allow 3D-reconstructions of β-amyloid plaques via a single scan of an uncropped brain. Ultramicroscopy makes this possible by replacing mechanical slicing in standard histology by optical sectioning. It allows a time efficient analysis of the amyloid plaque distribution in the entire mouse brain with 3D cellular resolution. We herein labeled β-amyloid deposits in a transgenic mouse model of cerebral β-amyloidosis (APPPS1 transgenic mice) with two intraperitoneal injections of the amyloid-binding fluorescent dye methoxy-X04. Upon postmortem analysis the total number of β-amyloid plaques, the β-amyloid load (volume percent) and the amyloid plaque size distributions were measured in the frontal cortex of two age groups (2.5 versus 7-8.5 month old mice). Applying ultramicroscopy we found in a proof-of-principle study that the number of β-amyloid plaques increases with age. In our experiments we further observed an increase of large plaques in the older age group of mice. We demonstrate that ultramicroscopy is a fast, and accurate analysis technique for studying β-amyloid lesions in transgenic mice allowing the 3D staging of β-amyloid plaque development. This in turn is the basis to study neural network degeneration upon cerebral β-amyloidosis and to assess Aβ-targeting therapeutics. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 542 | _ | _ | |i 2015-05-27 |2 Crossref |u http://creativecommons.org/licenses/by/4.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a 1,4-bis(4'-hydroxystyryl)-2-methoxybenzene |2 NLM Chemicals |
| 650 | _ | 7 | |a Alkenes |2 NLM Chemicals |
| 650 | _ | 7 | |a Amyloid beta-Protein Precursor |2 NLM Chemicals |
| 650 | _ | 7 | |a Benzene Derivatives |2 NLM Chemicals |
| 650 | _ | 7 | |a Fluorescent Dyes |2 NLM Chemicals |
| 650 | _ | 7 | |a Stilbenes |2 NLM Chemicals |
| 650 | _ | 2 | |a Alkenes: analysis |2 MeSH |
| 650 | _ | 2 | |a Alkenes: metabolism |2 MeSH |
| 650 | _ | 2 | |a Amyloid beta-Protein Precursor: genetics |2 MeSH |
| 650 | _ | 2 | |a Amyloidosis: pathology |2 MeSH |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Benzene Derivatives: analysis |2 MeSH |
| 650 | _ | 2 | |a Benzene Derivatives: metabolism |2 MeSH |
| 650 | _ | 2 | |a Brain: pathology |2 MeSH |
| 650 | _ | 2 | |a Disease Models, Animal |2 MeSH |
| 650 | _ | 2 | |a Fluorescent Dyes: analysis |2 MeSH |
| 650 | _ | 2 | |a Fluorescent Dyes: metabolism |2 MeSH |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Imaging, Three-Dimensional: methods |2 MeSH |
| 650 | _ | 2 | |a Mice, Inbred C57BL |2 MeSH |
| 650 | _ | 2 | |a Mice, Transgenic |2 MeSH |
| 650 | _ | 2 | |a Microscopy: methods |2 MeSH |
| 650 | _ | 2 | |a Plaque, Amyloid: pathology |2 MeSH |
| 650 | _ | 2 | |a Stilbenes |2 MeSH |
| 700 | 1 | _ | |a Becker, Klaus |b 1 |
| 700 | 1 | _ | |a Wegenast-Braun, Bettina M |0 P:(DE-2719)2814007 |b 2 |u dzne |
| 700 | 1 | _ | |a Grathwohl, Stefan A |0 P:(DE-2719)2501880 |b 3 |u dzne |
| 700 | 1 | _ | |a Jucker, Mathias |0 P:(DE-2719)2000010 |b 4 |u dzne |
| 700 | 1 | _ | |a Dodt, Hans-Ulrich |b 5 |
| 773 | 1 | 8 | |a 10.1371/journal.pone.0125418 |b : Public Library of Science (PLoS), 2015-05-27 |n 5 |p e0125418 |3 journal-article |2 Crossref |t PLOS ONE |v 10 |y 2015 |x 1932-6203 |
| 773 | _ | _ | |a 10.1371/journal.pone.0125418 |g Vol. 10, no. 5, p. e0125418 - |0 PERI:(DE-600)2267670-3 |n 5 |q 10:5 |t PLOS ONE |v 10 |y 2015 |x 1932-6203 |
| 856 | 4 | _ | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0125418 |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446269 |
| 856 | 4 | _ | |u https://pub.dzne.de/record/137945/files/4638.PDF |y OpenAccess |
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