%0 Journal Article
%A Schurig, Katja
%A Zieris, Andrea
%A Hermann, Andreas
%A Freudenberg, Uwe
%A Heidel, Sabine
%A Grimmer, Milauscha
%A Storch, Alexander
%A Werner, Carsten
%T Neurotropic growth factors and glycosaminoglycan based matrices to induce dopaminergic tissue formation.
%J Biomaterials
%V 67
%@ 0142-9612
%C Amsterdam [u.a.]
%I Elsevier Science
%M DZNE-2020-04433
%P 205-213
%D 2015
%X Current cell replacement therapies in Parkinson's disease (PD) are limited by low survival of transplanted cell and lacking regeneration of neuronal circuitries. Therefore, bioartificial cell carriers and growth/differentiation factors are applied to improve the integration of transplants and maximize newly generated and/or residual dopaminergic function. In this work, biohybrid poly(ethylene glycol) (starPEG)-heparin hydrogels releasing fibroblast growth factor 2 (FGF-2) and glial-derived neurotrophic factor (GDNF) were used to trigger dopaminergic tissue formation by primary murine midbrain cells in vitro. Matrix-delivered FGF-2 enhanced cell viability while release of GDNF had a pro-neuronal/dopaminergic effect. Combined delivery of both factors from the glycosaminoglycan-based matrices resulted in a tremendous improvement in survival and maturation capacity of dopaminergic neurons as obvious from tyrosine hydroxylase expression and neurite outgrowth. The reported data demonstrate that glycosaminoglycan-based hydrogels can facilitate the administration of neurotrophic factors and are therefore instrumental in potential future treatments of PD.
%K Animals
%K Cell Differentiation: drug effects
%K Cell Survival: drug effects
%K Cells, Cultured
%K Dopaminergic Neurons: cytology
%K Dopaminergic Neurons: drug effects
%K Drug Liberation
%K Female
%K Fetus: cytology
%K Fibroblast Growth Factor 2: pharmacology
%K Glial Cell Line-Derived Neurotrophic Factor: pharmacology
%K Glycosaminoglycans: pharmacology
%K Heparin: chemistry
%K Hydrogels: chemical synthesis
%K Mesencephalon: cytology
%K Mice, Inbred C57BL
%K Nerve Growth Factors: pharmacology
%K Neural Stem Cells: cytology
%K Neural Stem Cells: drug effects
%K Neurites: drug effects
%K Neurites: metabolism
%K Neuroglia: cytology
%K Neuroglia: drug effects
%K Polyethylene Glycols: chemical synthesis
%K Tissue Engineering: methods
%K Glial Cell Line-Derived Neurotrophic Factor (NLM Chemicals)
%K Glycosaminoglycans (NLM Chemicals)
%K Hydrogels (NLM Chemicals)
%K Nerve Growth Factors (NLM Chemicals)
%K Fibroblast Growth Factor 2 (NLM Chemicals)
%K Polyethylene Glycols (NLM Chemicals)
%K Heparin (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:26222283
%R 10.1016/j.biomaterials.2015.07.029
%U https://pub.dzne.de/record/138111