TY  - JOUR
AU  - Schurig, Katja
AU  - Zieris, Andrea
AU  - Hermann, Andreas
AU  - Freudenberg, Uwe
AU  - Heidel, Sabine
AU  - Grimmer, Milauscha
AU  - Storch, Alexander
AU  - Werner, Carsten
TI  - Neurotropic growth factors and glycosaminoglycan based matrices to induce dopaminergic tissue formation.
JO  - Biomaterials
VL  - 67
SN  - 0142-9612
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - DZNE-2020-04433
SP  - 205-213
PY  - 2015
AB  - Current cell replacement therapies in Parkinson's disease (PD) are limited by low survival of transplanted cell and lacking regeneration of neuronal circuitries. Therefore, bioartificial cell carriers and growth/differentiation factors are applied to improve the integration of transplants and maximize newly generated and/or residual dopaminergic function. In this work, biohybrid poly(ethylene glycol) (starPEG)-heparin hydrogels releasing fibroblast growth factor 2 (FGF-2) and glial-derived neurotrophic factor (GDNF) were used to trigger dopaminergic tissue formation by primary murine midbrain cells in vitro. Matrix-delivered FGF-2 enhanced cell viability while release of GDNF had a pro-neuronal/dopaminergic effect. Combined delivery of both factors from the glycosaminoglycan-based matrices resulted in a tremendous improvement in survival and maturation capacity of dopaminergic neurons as obvious from tyrosine hydroxylase expression and neurite outgrowth. The reported data demonstrate that glycosaminoglycan-based hydrogels can facilitate the administration of neurotrophic factors and are therefore instrumental in potential future treatments of PD.
KW  - Animals
KW  - Cell Differentiation: drug effects
KW  - Cell Survival: drug effects
KW  - Cells, Cultured
KW  - Dopaminergic Neurons: cytology
KW  - Dopaminergic Neurons: drug effects
KW  - Drug Liberation
KW  - Female
KW  - Fetus: cytology
KW  - Fibroblast Growth Factor 2: pharmacology
KW  - Glial Cell Line-Derived Neurotrophic Factor: pharmacology
KW  - Glycosaminoglycans: pharmacology
KW  - Heparin: chemistry
KW  - Hydrogels: chemical synthesis
KW  - Mesencephalon: cytology
KW  - Mice, Inbred C57BL
KW  - Nerve Growth Factors: pharmacology
KW  - Neural Stem Cells: cytology
KW  - Neural Stem Cells: drug effects
KW  - Neurites: drug effects
KW  - Neurites: metabolism
KW  - Neuroglia: cytology
KW  - Neuroglia: drug effects
KW  - Polyethylene Glycols: chemical synthesis
KW  - Tissue Engineering: methods
KW  - Glial Cell Line-Derived Neurotrophic Factor (NLM Chemicals)
KW  - Glycosaminoglycans (NLM Chemicals)
KW  - Hydrogels (NLM Chemicals)
KW  - Nerve Growth Factors (NLM Chemicals)
KW  - Fibroblast Growth Factor 2 (NLM Chemicals)
KW  - Polyethylene Glycols (NLM Chemicals)
KW  - Heparin (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:26222283
DO  - DOI:10.1016/j.biomaterials.2015.07.029
UR  - https://pub.dzne.de/record/138111
ER  -